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Literature summary extracted from
- Properties and applications of an immobilized mixed-function hepatic drug oxidase (1976), Methods Enzymol., 44, 849-856.
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.8 | Sus scrofa | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.13.8 | liver | - |
Sus scrofa | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.13.8 | glass-bead immobilized enzyme: 0-4°C, 0.025 M phosphate buffer, several months with little or no loss of activity | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.8 | additional information | catalyzes NADPH- and O2-dependent N-oxidation of N-substituted amines and hydrazines and the S-oxidation of thioureylenes and thiols | Sus scrofa | ? | - |
? |  |
| 1.14.13.8 | N,N-dimethylaniline + NADPH + O2 | - |
Sus scrofa | N,N-dimethylaniline N-oxide + NADP+ + H2O | - |
? | |
| 1.14.13.8 | secondary amine + NADPH + O2 | - |
Sus scrofa | secondary nitrone + NADP+ + H2O | first oxidation to the N-hydroxy amine and then to the corresponding nitrone | ? | |
| 1.14.13.8 | tertiary amine + NADPH + O2 | - |
Sus scrofa | tertiary N-oxide + NADP+ + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.8 | 25 | 28 | N,N-dimethylaniline, immobilized enzyme | Sus scrofa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.8 | 38 | - |
pH 7.6, half-life of free enzyme: 10 min, half-life of immobilized enzyme 5 h | Sus scrofa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.8 | 7.6 | - |
N,N-dimethylaniline, immobilized enzyme | Sus scrofa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.8 | NADH | concentration of NADPH required for half-maximal velocity is one-tenth of that for NADH | Sus scrofa | |
| 1.14.13.8 | NADPH | - |
Sus scrofa | |
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Release 2023.1 (January 2023)
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