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Literature summary extracted from

  • Ameyama, M.; Adachi, O.
    D-Fructose dehydrogenase from Gluconobacter industrius, membrane-bound (1982), Methods Enzymol., 89, 154-159.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.5.14 D-fructose, 5-10%, stabilizes Gluconobacter oxydans
1.1.5.14 glycerol, 5-10%, stabilizes Gluconobacter oxydans
1.1.5.14 sucrose, 5-10%, stabilizes Gluconobacter oxydans
1.1.5.14 Triton X-100 is essential for preservation of purified enzyme Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.14 10
-
 D-fructose
-
 Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.14 cytoplasmic membrane outer surface Gluconobacter oxydans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.14 19700
-
 1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE Gluconobacter oxydans
1.1.5.14 50800
-
 1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE Gluconobacter oxydans
1.1.5.14 67000
-
 1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE Gluconobacter oxydans
1.1.5.14 140000
-
 gel filtration Gluconobacter oxydans

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.14 Gluconobacter oxydans
-
 IFO 3260
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.14
-
 Gluconobacter oxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.14 172
-
-
 Gluconobacter oxydans

Storage Stability

EC Number Storage Stability Organism
1.1.5.14 -20°C or below, activity completely preserved Gluconobacter oxydans
1.1.5.14 0-4°C, pH 4.0-5.0, 0.1% Triton X-100, 1 mM 2-mercaptoethanol, for at least 2 weeks Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.14 D-fructose + 2,6-dichlorophenolindophenol
-
 Gluconobacter oxydans 5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.14 D-fructose + ferricyanide
-
 Gluconobacter oxydans 5-dehydro-D-fructose + ferrocyanide
-
 ?
1.1.5.14 D-fructose + nitro blue tetrazolium
-
 Gluconobacter oxydans 5-dehydro-D-fructose + ?
-
 ?
1.1.5.14 D-fructose + phenazine methosulfate
-
 Gluconobacter oxydans 5-dehydro-D-fructose + ?
-
 ?
1.1.5.14 additional information no activity with NADP+ Gluconobacter oxydans ?
-
 ?
1.1.5.14 additional information no activity with O2 Gluconobacter oxydans ?
-
 ?
1.1.5.14 additional information no activity with NAD+ Gluconobacter oxydans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.14 trimer 1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.14 25
-
-
 Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.14 4
-
-
 Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.14 cytochrome c enzyme contains a cytochrome c of 50800 Da Gluconobacter oxydans