BRENDA - Enzyme Database

Purification and properties of secondary alcohol oxidase from a strain of Pseudomonas

Morita, M.; Hamada, N.; Sakai, K.; Watanabe, Y.; Agric. Biol. Chem. 43, 1225-1235 (1979)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.18
EDTA
weak inhibition
Pseudomonas sp.
1.1.3.18
Hg2+
weak inhibition
Pseudomonas sp.
1.1.3.18
o-phenanthroline
weak inhibition
[Pseudomonas] boreopolis
1.1.3.18
o-phenanthroline
-
Pseudomonas sp.
1.1.3.18
Pb2+
weak inhibition
Pseudomonas sp.
1.1.3.18
Zn2+
weak inhibition
Pseudomonas sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.3.18
Co2+
catalyzes the decompositon of H2O2
Pseudomonas sp.
1.1.3.18
Cu2+
catalyzes the decompositon of H2O2
Pseudomonas sp.
1.1.3.18
Fe2+
contains 1 atom non-heme iron per molecule, catalyzes the decomposition of H2O2
Pseudomonas sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.18
49500
-
sedimentation equilibrium
Pseudomonas sp.
1.1.3.18
50000
-
gel filtration
Pseudomonas sp.
1.1.3.18
50500
-
SDS-PAGE
Pseudomonas sp.
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.3.18
Pseudomonas sp.
-
unidentified strain
-
1.1.3.18
[Pseudomonas] boreopolis
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.3.18
-
[Pseudomonas] boreopolis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.3.18
1.5
-
-
Pseudomonas sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.3.18
1,2,6-hexanetriol + O2
-
348177
Pseudomonas sp.
1,2,6-hexanetrione + H2O2
-
-
-
?
1.1.3.18
1,2-hexanediol + O2
-
348177
Pseudomonas sp.
1,2-hexanedione + H2O2
-
-
-
?
1.1.3.18
1,2-propanediol + O2
-
348177
Pseudomonas sp.
1,2-propanedione + H2O2
-
-
-
?
1.1.3.18
1,3-cyclohexanediol + O2
-
348177
Pseudomonas sp.
1,3-cyclohexanedione + H2O2
-
-
-
?
1.1.3.18
1,4-benzenedimethanol + O2
-
348177
Pseudomonas sp.
benzene-1,4-dicarbaldehyde + H2O2
-
-
-
?
1.1.3.18
1,5-pentanediol + O2
-
348177
Pseudomonas sp.
1,5-pentanedione + H2O2
-
-
-
?
1.1.3.18
1-decanol + O2
-
348177
Pseudomonas sp.
n-decanal + H2O2
-
-
-
?
1.1.3.18
1-heptanol + O2
-
348177
Pseudomonas sp.
n-heptanal + H2O2
-
-
-
?
1.1.3.18
1-pentanol + O2
-
348177
Pseudomonas sp.
n-pentanal + H2O2
-
-
-
?
1.1.3.18
1-propanol + O2
-
348177
Pseudomonas sp.
n-propanal + H2O2
-
-
-
?
1.1.3.18
2,5-hexanediol + O2
-
348177
Pseudomonas sp.
2,5-hexanedione + H2O2
-
-
-
?
1.1.3.18
2-butanol + O2
-
348177
Pseudomonas sp.
2-butanone + H2O2
-
-
-
?
1.1.3.18
2-decanol + O2
-
348177
Pseudomonas sp.
2-decanone + H2O2
-
-
-
?
1.1.3.18
2-heptanol + O2
-
348177
Pseudomonas sp.
2-heptanone + H2O2
-
-
-
?
1.1.3.18
2-hexanol + O2
-
348177
Pseudomonas sp.
2-hexanone + H2O2
-
-
-
?
1.1.3.18
2-nonanol + O2
-
348177
Pseudomonas sp.
2-nonanone + H2O2
-
-
-
?
1.1.3.18
2-octanol + O2
-
348177
Pseudomonas sp.
2-octanone + H2O2
-
-
-
?
1.1.3.18
2-pentanol + O2
-
348177
Pseudomonas sp.
2-pentanone + H2O2
-
-
-
?
1.1.3.18
2-propanol + O2
-
348177
Pseudomonas sp.
2-propanone + H2O2
-
-
-
?
1.1.3.18
3-heptanol + O2
-
348177
Pseudomonas sp.
3-heptanone + H2O2
-
-
-
?
1.1.3.18
3-hexanol + O2
-
348177
Pseudomonas sp.
3-hexanone + H2O2
-
-
-
?
1.1.3.18
3-nonanol + O2
-
348177
Pseudomonas sp.
3-nonanone + H2O2
-
-
-
?
1.1.3.18
3-octanol + O2
-
348177
Pseudomonas sp.
3-octanone + H2O2
-
-
-
?
1.1.3.18
3-pentanol + O2
-
348177
Pseudomonas sp.
3-pentanone + H2O2
-
-
-
?
1.1.3.18
4-decanol + O2
-
348177
Pseudomonas sp.
4-decanone + H2O2
-
-
-
?
1.1.3.18
4-heptanol + O2
-
348177
Pseudomonas sp.
4-heptanone + H2O2
-
-
-
?
1.1.3.18
4-heptanol + O2
-
348177
[Pseudomonas] boreopolis
4-heptanone + H2O2
-
-
-
?
1.1.3.18
4-nonanol + O2
-
348177
Pseudomonas sp.
4-nonanone + H2O2
-
-
-
?
1.1.3.18
4-octanol + O2
-
348177
Pseudomonas sp.
4-octanone + H2O2
-
-
-
?
1.1.3.18
5-decanol + O2
-
348177
Pseudomonas sp.
5-decanone + H2O2
-
-
-
?
1.1.3.18
5-nonanol + O2
-
348177
Pseudomonas sp.
5-nonanone + H2O2
-
-
-
?
1.1.3.18
6-undecanol + O2
-
348177
Pseudomonas sp.
6-undecanone + H2O2
-
-
-
?
1.1.3.18
benzyl alcohol + O2
-
348177
Pseudomonas sp.
benzaldehyde + H2O2
-
-
-
?
1.1.3.18
cyclohexanol + O2
-
348177
Pseudomonas sp.
cyclohexanone + H2O2
-
-
-
?
1.1.3.18
polyvinyl alcohol + O2
-
348177
Pseudomonas sp.
polyvinylketone + H2O2
-
-
-
?
1.1.3.18
polyvinyl alcohol + O2
-
348177
[Pseudomonas] boreopolis
polyvinylketone + H2O2
-
-
-
?
1.1.3.18
sec-phenethyl alcohol + O2
-
348177
Pseudomonas sp.
1-phenylethanone + H2O2
-
-
-
?
1.1.3.18
tert-butanol + O2
-
348177
Pseudomonas sp.
tert-butanone + H2O2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.3.18
monomer
1 * 50500, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.3.18
50
-
-
Pseudomonas sp.
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.3.18
20
60
-
Pseudomonas sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.3.18
50
-
stable below
Pseudomonas sp.
1.1.3.18
70
-
activity remains 100% below 50°C, completely lost at 70°C
Pseudomonas sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.18
7
-
-
Pseudomonas sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.3.18
5
10
-
Pseudomonas sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.3.18
4.5
9
stable between
[Pseudomonas] boreopolis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.18
EDTA
weak inhibition
Pseudomonas sp.
1.1.3.18
Hg2+
weak inhibition
Pseudomonas sp.
1.1.3.18
o-phenanthroline
-
Pseudomonas sp.
1.1.3.18
o-phenanthroline
weak inhibition
[Pseudomonas] boreopolis
1.1.3.18
Pb2+
weak inhibition
Pseudomonas sp.
1.1.3.18
Zn2+
weak inhibition
Pseudomonas sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.3.18
Co2+
catalyzes the decompositon of H2O2
Pseudomonas sp.
1.1.3.18
Cu2+
catalyzes the decompositon of H2O2
Pseudomonas sp.
1.1.3.18
Fe2+
contains 1 atom non-heme iron per molecule, catalyzes the decomposition of H2O2
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.18
49500
-
sedimentation equilibrium
Pseudomonas sp.
1.1.3.18
50000
-
gel filtration
Pseudomonas sp.
1.1.3.18
50500
-
SDS-PAGE
Pseudomonas sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.3.18
-
[Pseudomonas] boreopolis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.3.18
1.5
-
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.3.18
1,2,6-hexanetriol + O2
-
348177
Pseudomonas sp.
1,2,6-hexanetrione + H2O2
-
-
-
?
1.1.3.18
1,2-hexanediol + O2
-
348177
Pseudomonas sp.
1,2-hexanedione + H2O2
-
-
-
?
1.1.3.18
1,2-propanediol + O2
-
348177
Pseudomonas sp.
1,2-propanedione + H2O2
-
-
-
?
1.1.3.18
1,3-cyclohexanediol + O2
-
348177
Pseudomonas sp.
1,3-cyclohexanedione + H2O2
-
-
-
?
1.1.3.18
1,4-benzenedimethanol + O2
-
348177
Pseudomonas sp.
benzene-1,4-dicarbaldehyde + H2O2
-
-
-
?
1.1.3.18
1,5-pentanediol + O2
-
348177
Pseudomonas sp.
1,5-pentanedione + H2O2
-
-
-
?
1.1.3.18
1-decanol + O2
-
348177
Pseudomonas sp.
n-decanal + H2O2
-
-
-
?
1.1.3.18
1-heptanol + O2
-
348177
Pseudomonas sp.
n-heptanal + H2O2
-
-
-
?
1.1.3.18
1-pentanol + O2
-
348177
Pseudomonas sp.
n-pentanal + H2O2
-
-
-
?
1.1.3.18
1-propanol + O2
-
348177
Pseudomonas sp.
n-propanal + H2O2
-
-
-
?
1.1.3.18
2,5-hexanediol + O2
-
348177
Pseudomonas sp.
2,5-hexanedione + H2O2
-
-
-
?
1.1.3.18
2-butanol + O2
-
348177
Pseudomonas sp.
2-butanone + H2O2
-
-
-
?
1.1.3.18
2-decanol + O2
-
348177
Pseudomonas sp.
2-decanone + H2O2
-
-
-
?
1.1.3.18
2-heptanol + O2
-
348177
Pseudomonas sp.
2-heptanone + H2O2
-
-
-
?
1.1.3.18
2-hexanol + O2
-
348177
Pseudomonas sp.
2-hexanone + H2O2
-
-
-
?
1.1.3.18
2-nonanol + O2
-
348177
Pseudomonas sp.
2-nonanone + H2O2
-
-
-
?
1.1.3.18
2-octanol + O2
-
348177
Pseudomonas sp.
2-octanone + H2O2
-
-
-
?
1.1.3.18
2-pentanol + O2
-
348177
Pseudomonas sp.
2-pentanone + H2O2
-
-
-
?
1.1.3.18
2-propanol + O2
-
348177
Pseudomonas sp.
2-propanone + H2O2
-
-
-
?
1.1.3.18
3-heptanol + O2
-
348177
Pseudomonas sp.
3-heptanone + H2O2
-
-
-
?
1.1.3.18
3-hexanol + O2
-
348177
Pseudomonas sp.
3-hexanone + H2O2
-
-
-
?
1.1.3.18
3-nonanol + O2
-
348177
Pseudomonas sp.
3-nonanone + H2O2
-
-
-
?
1.1.3.18
3-octanol + O2
-
348177
Pseudomonas sp.
3-octanone + H2O2
-
-
-
?
1.1.3.18
3-pentanol + O2
-
348177
Pseudomonas sp.
3-pentanone + H2O2
-
-
-
?
1.1.3.18
4-decanol + O2
-
348177
Pseudomonas sp.
4-decanone + H2O2
-
-
-
?
1.1.3.18
4-heptanol + O2
-
348177
Pseudomonas sp.
4-heptanone + H2O2
-
-
-
?
1.1.3.18
4-heptanol + O2
-
348177
[Pseudomonas] boreopolis
4-heptanone + H2O2
-
-
-
?
1.1.3.18
4-nonanol + O2
-
348177
Pseudomonas sp.
4-nonanone + H2O2
-
-
-
?
1.1.3.18
4-octanol + O2
-
348177
Pseudomonas sp.
4-octanone + H2O2
-
-
-
?
1.1.3.18
5-decanol + O2
-
348177
Pseudomonas sp.
5-decanone + H2O2
-
-
-
?
1.1.3.18
5-nonanol + O2
-
348177
Pseudomonas sp.
5-nonanone + H2O2
-
-
-
?
1.1.3.18
6-undecanol + O2
-
348177
Pseudomonas sp.
6-undecanone + H2O2
-
-
-
?
1.1.3.18
benzyl alcohol + O2
-
348177
Pseudomonas sp.
benzaldehyde + H2O2
-
-
-
?
1.1.3.18
cyclohexanol + O2
-
348177
Pseudomonas sp.
cyclohexanone + H2O2
-
-
-
?
1.1.3.18
polyvinyl alcohol + O2
-
348177
Pseudomonas sp.
polyvinylketone + H2O2
-
-
-
?
1.1.3.18
polyvinyl alcohol + O2
-
348177
[Pseudomonas] boreopolis
polyvinylketone + H2O2
-
-
-
?
1.1.3.18
sec-phenethyl alcohol + O2
-
348177
Pseudomonas sp.
1-phenylethanone + H2O2
-
-
-
?
1.1.3.18
tert-butanol + O2
-
348177
Pseudomonas sp.
tert-butanone + H2O2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.3.18
monomer
1 * 50500, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.3.18
50
-
-
Pseudomonas sp.
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.3.18
20
60
-
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.3.18
50
-
stable below
Pseudomonas sp.
1.1.3.18
70
-
activity remains 100% below 50°C, completely lost at 70°C
Pseudomonas sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.18
7
-
-
Pseudomonas sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.3.18
5
10
-
Pseudomonas sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.3.18
4.5
9
stable between
[Pseudomonas] boreopolis