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Literature summary extracted from

  • Fujimori, K.; Okada, T.; Urade, Y.
    Expression of NADP(+)-Dependent 15-Hydroxyprostaglandin Dehydrogenase mRNA in Monkey Ocular Tissues and Characterization of Its Recombinant Enzyme (2002), J. Biochem., 131, 383-389.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.141 cloning of cDNA Macaca fascicularis
1.1.1.197
-
 Macaca fascicularis
1.1.1.231
-
 Macaca fascicularis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.141 28900
-
 2 * 28900, deduced from nucleotide sequence Macaca fascicularis
1.1.1.197 30500
-
 x * 30500, deduced from gene sequence, x * 31000, SDS-PAGE Macaca fascicularis
1.1.1.197 31000
-
 x * 30500, deduced from gene sequence, x * 31000, SDS-PAGE Macaca fascicularis
1.1.1.231 31000
-
 SDs-PAGE of recombinant enzyme Macaca fascicularis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.141 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+ Macaca fascicularis
-
 (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.141 Macaca fascicularis
-
-
-
1.1.1.197 Macaca fascicularis Q8MI29
-
-
1.1.1.231 Macaca fascicularis
-
 monkey
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.141 cornea low expression Macaca fascicularis
-
1.1.1.141 liver
-
 Macaca fascicularis
-
1.1.1.141 lung
-
 Macaca fascicularis
-
1.1.1.197 eye epithelial cells of cornea Macaca fascicularis
-
1.1.1.231 eye
-
 Macaca fascicularis
-
1.1.1.231 additional information tissue distribution of m-RNA Macaca fascicularis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.197 0.0012
-
 substrate latanoprost, pH 7.0, 25°C Macaca fascicularis
1.1.1.197 0.0022
-
 substrate acid form of latanoprost, pH 7.0, 25°C Macaca fascicularis
1.1.1.197 0.0061
-
 substrate prostaglandin F2alpha, cosubstrate NAD+, pH 7.0, 25°C Macaca fascicularis
1.1.1.197 0.0098
-
 substrate prostaglandin E2, cosubstrate NADP+, pH 7.0, 25°C Macaca fascicularis
1.1.1.197 0.0243
-
 substrate prostaglandin F2alpha, cosubstrate NADP+, pH 7.0, 25°C Macaca fascicularis
1.1.1.231 1.217
-
 recombinant enzyme, latanoprost + NADP+ Macaca fascicularis
1.1.1.231 2.17
-
 recombinant enzyme, acid form of latanoprost + NADP+ Macaca fascicularis
1.1.1.231 6.08
-
 recombinant enzyme, prostaglandin F2alpha + NAD+ Macaca fascicularis
1.1.1.231 9.8
-
 recombinant enzyme, prostaglandin E2 + NADP+ Macaca fascicularis
1.1.1.231 24.3
-
 recombinant enzyme, prostaglandin F2alpha + NADP+ Macaca fascicularis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.141 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate + NAD+
-
 Macaca fascicularis (5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NADH + H+
-
 ?
1.1.1.197 acid form of latanoprost + NADP+
-
 Macaca fascicularis ? + NADPH
-
 ?
1.1.1.197 latanoprost + NADP+
-
 Macaca fascicularis ? + NADPH
-
 ?
1.1.1.197 prostaglandin E2 + NADPH + H+
-
 Macaca fascicularis prostaglandin F2alpha + NADP+
-
 ?
1.1.1.197 prostaglandin F2alpha + NADP+
-
 Macaca fascicularis prostaglandin E1 + NADPH
-
 ?
1.1.1.231 prostaglandin E2 + NADP+
-
 Macaca fascicularis 15-ketoprostaglandin E2 + NADPH + H+
-
 ?
1.1.1.231 prostaglandin F2alpha + NAD+
-
 Macaca fascicularis 15-ketoprostaglandin F2alpha + NADH + H+
-
 ?
1.1.1.231 prostaglandin F2alpha + NADP+
-
 Macaca fascicularis 15-ketoprostaglandin F2alpha + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.141 dimer 2 * 28900, deduced from nucleotide sequence Macaca fascicularis
1.1.1.197 ? x * 30500, deduced from gene sequence, x * 31000, SDS-PAGE Macaca fascicularis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.141 NAD+ dependent PGDH
-
 Macaca fascicularis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.197 NADP+
-
 Macaca fascicularis