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Literature summary extracted from

  • Stafford, H.A.; Lester, H.H.
    Flavan-3-ol biosynthesis. The conversion of (+)-dihydroquercetin and flavan-3,4-cis-diol (leucocyanidin) to (+)-catechin by reductases extracted from cell suspension cultures of douglas fir (1984), Plant Physiol., 76, 184-186.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.219 0.037
-
 (+)-dihydroquercetin estimation Pseudotsuga menziesii
1.17.1.3 0.037
-
 dihydroquercetin pH 7.4 Pseudotsuga menziesii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.3 2,3-trans-3,4-cis-leucocyanidin + NADPH Pseudotsuga menziesii proanthocyanidin biosynthesis (2R,3S)-catechin + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.219 Pseudotsuga menziesii
-
 douglas fir
-
1.17.1.3 Pseudotsuga menziesii
-
 douglas fir
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.219 callus
-
 Pseudotsuga menziesii
-
1.1.1.219 cell suspension culture
-
 Pseudotsuga menziesii
-
1.17.1.3 cell suspension culture
-
 Pseudotsuga menziesii
-
1.17.1.3 needle
-
 Pseudotsuga menziesii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.219 (+)-dihydroquercetin + NADPH
-
 Pseudotsuga menziesii cis-3,4-leucocyanidin + NADP+ i.e. 5,7,3',4'-tetrahydroxyflavan-3,4-cis-diol, 2,3-trans-configuration retained ?
1.17.1.3 2,3-trans-3,4-cis-leucocyanidin + NADPH enzyme acts mainly on the 3,4-cis rather than the 3,4-trans isomer Pseudotsuga menziesii (2R,3S)-catechin + NADP+ + H2O
-
 ?
1.17.1.3 2,3-trans-3,4-cis-leucocyanidin + NADPH proanthocyanidin biosynthesis Pseudotsuga menziesii (2R,3S)-catechin + NADP+ + H2O
-
 ?
1.17.1.3 dihydroquercetin + NADPH double step reduction Pseudotsuga menziesii (2R,3S)-catechin + NADP+ + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.3 7.4
-
-
 Pseudotsuga menziesii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.219 6.4 8 no significant variations in activity Pseudotsuga menziesii
1.17.1.3 6.4 8 no significant changes between pH 6.4 and pH 8.0 Pseudotsuga menziesii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.219 NADPH
-
 Pseudotsuga menziesii
1.17.1.3 NADPH dependent Pseudotsuga menziesii