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Literature summary extracted from

  • Hampton, A.; Nomura, A.
    Inosine 5-phosphate dehydrogenase. Site of inhibition by guanosine 5-phosphate and of inactivation by 6-chloro- and 6-mercaptopurine ribonucleoside 5-phosphates (1967), Biochemistry, 6, 679-689.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.205 bovine serum albumin final concentration of 0.05% stimulates 50% Klebsiella aerogenes
1.1.1.205 glutathione level for maximum activity: 2-4 mM Klebsiella aerogenes

General Stability

EC Number General Stability Organism
1.1.1.205 activity is maintained when stored for 3 weeks at -20°C, during which it can be thawed and frozen twice and kept at 2°C for two 24-h periods Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.205 6-Chloropurine ribonucleoside 5'-phosphate inosine 5'-phosphate and guanosine 5'-phosphate retard inactivation Klebsiella aerogenes
1.1.1.205 6-thioinosine 5'-phosphate complete inactivation after 10 min preincubation at a concentration of 0.001 mM; in absence of glutathione Klebsiella aerogenes
1.1.1.205 glutathione above 4 mM Klebsiella aerogenes
1.1.1.205 GMP competitive with respect to IMP Klebsiella aerogenes
1.1.1.205 K+ above 0.1 M Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.205 0.02
-
 6-thio-IMP pH 8.1 Klebsiella aerogenes
1.1.1.205 0.021
-
 inosine 5'-phosphate 6-thioinosine 5'-phosphate, constant in pH-range 6.5-8.2, sharp decrease at higher pH Klebsiella aerogenes
1.1.1.205 1.1
-
 NAD+
-
 Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.205 K+ requirement of 0.1 M for maximal activity Klebsiella aerogenes
1.1.1.205 NH4+ required Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.205 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.205
-
 Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.205 17.2
-
-
 Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
1.1.1.205 2°C, 50% loss of activity after 2-3 days in dilutions with water Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.205 6-thio-IMP + NAD+ + H2O
-
 Klebsiella aerogenes 6-thio-XMP + NADH + H+
-
 r
1.1.1.205 6-thioinosine 5'-phosphate + NAD+ + H2O
-
 Klebsiella aerogenes 6-thioxanthosine 5'-phosphate + NADH
-
 ?
1.1.1.205 inosine 5'-phosphate + NAD+ + H2O
-
 Klebsiella aerogenes xanthosine 5'-phosphate + NADH
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.205 8.2
-
-
 Klebsiella aerogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.205 NAD+
-
 Klebsiella aerogenes

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.205 0.13
-
 GMP
-
 Klebsiella aerogenes
1.1.1.205 0.14
-
 6-thio-IMP
-
 Klebsiella aerogenes