Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schieber, A.; Frank, R.W.; Ghisla, S.
    Purification and properties of prostaglandin 9-ketoreductase from pig and human kidney. Identity with human carbonyl reductase (1992), Eur. J. Biochem., 206, 491-502.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.184 0.002
-
 9,10-phenanthrenquinone
-
 Sus scrofa
1.1.1.184 0.16
-
 prostaglandin E2
-
 Sus scrofa
1.1.1.184 0.31
-
 menadione
-
 Sus scrofa
1.1.1.184 0.45
-
 4-nitrobenzaldehyde
-
 Sus scrofa
1.1.1.189 0.0023
-
 9,10-phenanthrenequinone
-
 Sus scrofa
1.1.1.189 0.0052
-
 NADPH
-
 Sus scrofa
1.1.1.189 0.16
-
 prostaglandin E2
-
 Sus scrofa
1.1.1.189 0.31
-
 menadione
-
 Sus scrofa
1.1.1.189 0.45
-
 4-nitrobenzaldehyde
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.184 Zinc
-
 Homo sapiens
1.1.1.184 Zinc
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.184 additional information
-
 amino acid composition Homo sapiens
1.1.1.184 additional information
-
 amino acid composition Sus scrofa
1.1.1.184 28000 32000 gel filtration, SDS-PAGE Sus scrofa
1.1.1.184 34000
-
 gel filtration, SDS-PAGE Homo sapiens
1.1.1.189 28000
-
 gel filtration, 4 isoforms Sus scrofa
1.1.1.189 32000
-
 alpha, 1 * 32000, SDS-PAGE Sus scrofa
1.1.1.189 34000
-
 alpha, 1 * 34000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Homo sapiens
-
-
-
1.1.1.184 Sus scrofa
-
-
-
1.1.1.189 Homo sapiens
-
-
-
1.1.1.189 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.184
-
 Homo sapiens
1.1.1.184
-
 Sus scrofa
1.1.1.189
-
 Homo sapiens
1.1.1.189
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.184 kidney
-
 Homo sapiens
-
1.1.1.184 kidney
-
 Sus scrofa
-
1.1.1.189 kidney
-
 Homo sapiens
-
1.1.1.189 kidney
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.189 0.02
-
-
 Sus scrofa
1.1.1.189 0.034
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 4-nitrobenzaldehyde + NADPH
-
 Homo sapiens 4-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.184 4-nitrobenzaldehyde + NADPH
-
 Sus scrofa 4-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.184 9,10-phenanthrenequinone + NADPH
-
 Homo sapiens ?
-
 ?
1.1.1.184 9,10-phenanthrenequinone + NADPH
-
 Sus scrofa ?
-
 ?
1.1.1.184 menadione + NADPH
-
 Homo sapiens ?
-
 ?
1.1.1.184 menadione + NADPH
-
 Sus scrofa ?
-
 ?
1.1.1.184 additional information
-
 Homo sapiens ?
-
 ?
1.1.1.184 additional information catalyzes pro-S stereoselective hydrogen transfer from NADPH Sus scrofa ?
-
 ?
1.1.1.184 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Homo sapiens NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.1.184 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Sus scrofa NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.1.184 phenylglyoxal + NADPH
-
 Homo sapiens ?
-
 ?
1.1.1.184 phenylglyoxal + NADPH
-
 Sus scrofa ?
-
 ?
1.1.1.184 prostaglandin E2 + NADPH + H+
-
 Sus scrofa prostaglandin F2alpha + NADP+
-
 r
1.1.1.189 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NAD(P)+
-
 Homo sapiens (5Z,13E)-11alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H
-
 ?
1.1.1.189 (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NAD(P)+
-
 Sus scrofa (5Z,13E)-11alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H
-
 ir
1.1.1.189 (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 Homo sapiens (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NADPH + H+
-
 ?
1.1.1.189 (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 Homo sapiens (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NADPH + H+
-
 r
1.1.1.189 (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ enzyme is pro(S) stereo selective with regard to hydrogen transfer from NADPH Sus scrofa (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate + NADPH + H+
-
 r
1.1.1.189 (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 Homo sapiens (5Z,13E)-9alpha,11alpha-dihydroxy-15-oxoprosta-5,13-dienoate + NADPH + H+
-
 ?
1.1.1.189 (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 Sus scrofa (5Z,13E)-9alpha,11alpha-dihydroxy-15-oxoprosta-5,13-dienoate + NADPH + H+
-
 ir
1.1.1.189 (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADP+
-
 Homo sapiens (5Z,13E)-9alpha-hydroxy-11,15-dioxoprosta-5,13-dienoate + NADPH
-
 ?
1.1.1.189 (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADP+
-
 Sus scrofa (5Z,13E)-9alpha-hydroxy-11,15-dioxoprosta-5,13-dienoate + NADPH
-
 ir
1.1.1.189 17alpha-hydroxyprogesterone + NADPH
-
 Sus scrofa ?
-
 ?
1.1.1.189 2,6-dichloroindophenol + NADPH
-
 Sus scrofa ?
-
 ?
1.1.1.189 4-nitrobenzaldehyde + NADPH
-
 Sus scrofa ?
-
 ?
1.1.1.189 5alpha-dihydrotestosterone + NADPH
-
 Sus scrofa ?
-
 ?
1.1.1.189 9,10-phenanthrenequinone + NADPH
-
 Sus scrofa ?
-
 ?
1.1.1.189 alpha-tocopherolquinone + NADPH
-
 Sus scrofa ?
-
 ?
1.1.1.189 corticosterone + NADPH + H+
-
 Sus scrofa ?
-
 ?
1.1.1.189 cortisole + NADPH
-
 Sus scrofa ?
-
 ?
1.1.1.189 menadione + NADPH
-
 Sus scrofa ?
-
 ?
1.1.1.189 phenylglyoxal + NADPH
-
 Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.189 monomer alpha, 1 * 34000, SDS-PAGE Homo sapiens
1.1.1.189 monomer alpha, 1 * 32000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.189 37
-
-
 Homo sapiens
1.1.1.189 37
-
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.189 7.8 9
-
 Sus scrofa
1.1.1.189 8
-
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.189 NADPH
-
 Homo sapiens
1.1.1.189 NADPH
-
 Sus scrofa