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Literature summary extracted from

  • Staton, A.L.; Mazelis, M.
    The C-S lyases of higher plants: homogeneous beta-cystathionase of spinach leaves (1991), Arch. Biochem. Biophys., 290, 46-50.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.4.1.13 inactivated by repeated freezing and thawing Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.13 0.3
-
 L-cystathionine
-
 Spinacia oleracea
4.4.1.13 0.35
-
 L-djenkolate
-
 Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.13 53000
-
 4 * 53000, SDS-PAGE Spinacia oleracea
4.4.1.13 210000
-
 gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Spinacia oleracea
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.4.1.13 no modification enzyme is not a glycoprotein Spinacia oleracea

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.13 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.13 1.226
-
-
 Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
4.4.1.13 -10°C, stable for 1 month Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 L-cystathionine + H2O
-
 Spinacia oleracea L-homocysteine + NH3 + pyruvate
-
 ?
4.4.1.13 L-cystine + H2O 17% of the activity with L-cystathionine Spinacia oleracea L-thiocysteine + pyruvate + NH3
-
 ?
4.4.1.13 L-djenkolate + H2O 126% of the activity with L-cystathionine Spinacia oleracea pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.13 tetramer 4 * 53000, SDS-PAGE Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.13 8.6 8.7
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.13 pyridoxal 5'-phosphate required as cofactor Spinacia oleracea