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Literature summary extracted from

  • Nishizuka, Y.
    S-Alkyl-L-cysteine Lyase (Pseudomonas) (1971), Methods Enzymol., 17B, 470-474.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.13 S-methyl-L-cysteine substrate inhibition, when concentration exceeds 10 mM Pseudomonas cruciviae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.13 0.008
-
 pyridoxal phosphate
-
 Pseudomonas cruciviae
4.4.1.13 2.3
-
 S-methyl-L-cysteine
-
 Pseudomonas cruciviae
4.4.1.13 2.3
-
 S-methyl-L-cysteine sulfoxide
-
 Pseudomonas cruciviae

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Pseudomonas cruciviae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13
-
 Pseudomonas cruciviae

Storage Stability

EC Number Storage Stability Organism
4.4.1.13 -20°C, almost all activity lost after two weeks Pseudomonas cruciviae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 S-methyl-L-cysteine
-
 Pseudomonas cruciviae methyl mercaptane + pyruvate + NH3
-
 ir
4.4.1.13 S-methyl-L-cysteine sulfoxide
-
 Pseudomonas cruciviae methyl methanethiolsulfinate + pyruvate + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.13 23
-
-
 Pseudomonas cruciviae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.13 8.8
-
-
 Pseudomonas cruciviae

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.13 pyridoxal 5'-phosphate
-
 Pseudomonas cruciviae