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Literature summary extracted from

  • Manabe, T.; Hasumi, A.; Sugiyama, M.; Yamazaki, M.; Saito, K.
    Alliinase [S-alk(en)yl-L-cysteine sulfoxide lyase] from Allium tuberosum (Chinese chive): purification, localization, cDNA cloning and heterologous functional expression (1998), Eur. J. Biochem., 257, 21-30.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.4 expression in Escherichia coli and Saccharomyces cerevisiae Allium tuberosum

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.4 K280A almost no detectable alliinase activity Allium tuberosum
4.4.1.4 K280R almost no detectable alliinase activity Allium tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.4 2.7
-
 S-ethyl-L-Cys sulfoxide
-
 Allium tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.4 48000
-
 x * 48000, SDS-PAGE Allium tuberosum
4.4.1.4 54083
-
 x * 54083, calculation from nucleotide sequence Allium tuberosum
4.4.1.4 67000
-
 gel filtration Allium tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.4 additional information Allium tuberosum the enzyme is responsible for the degradative conversion of S-alk(en)yl-L-Cys sulfoxide to volatile odorous sulfur-containing metabolites ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.4 Allium tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.4
-
 Allium tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.4 shoot the enzyme predominantly accumulates in the bundle sheath cells Allium tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.4 152.8
-
-
 Allium tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.4 2 S-ethyl-L-Cys sulfoxide + H2O
-
 Allium tuberosum ethyl ethanethiosulfinate + 2 pyruvate + 2 NH3
-
 ?
4.4.1.4 additional information the enzyme is responsible for the degradative conversion of S-alk(en)yl-L-Cys sulfoxide to volatile odorous sulfur-containing metabolites Allium tuberosum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.4 ? x * 48000, SDS-PAGE Allium tuberosum
4.4.1.4 ? x * 54083, calculation from nucleotide sequence Allium tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.4 6.5 7 sodium phosphate buffer Allium tuberosum
4.4.1.4 8 8.5 Tris/HCl buffer Allium tuberosum

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.4 pyridoxal 5'-phosphate
-
 Allium tuberosum