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Literature summary extracted from

  • Stone, R.L.; Zalkin, H.; Dixon, J.E.
    Expression, purification, and kinetic characterization of recombinant human adenylosuccinate lyase (1993), J. Biol. Chem., 268, 19710-19716.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.2 normal and mutant enzyme S413P, expression in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.2.2 AMP
-
 Homo sapiens
4.3.2.2 fumarate noncompetitive Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.2 additional information
-
 additional information pH-dependence of Km-value Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.2 50000
-
 4 * 50000, SDS-PAGE Homo sapiens
4.3.2.2 196000
-
 wild-type enzyme, gel filtration Homo sapiens
4.3.2.2 201000
-
 mutant enzyme S413P, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.2.2 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide Homo sapiens reaction in the biosynthesis of AMP 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
4.3.2.2 N6-(1,2-dicarboxyethyl)AMP Homo sapiens reaction in the biosynthesis of AMP fumarate + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.2 Homo sapiens
-
 normal and mutant enzyme S413P
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.2 normal and mutant enzyme S413P Homo sapiens

Storage Stability

EC Number Storage Stability Organism
4.3.2.2 -80°C or -20°C, recombinant enzyme is very unstable regardless of salt or glycerol concentration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.2 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide reaction in the biosynthesis of AMP Homo sapiens 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
4.3.2.2 N6-(1,2-dicarboxyethyl)AMP reaction in the biosynthesis of AMP Homo sapiens fumarate + AMP
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.2.2 tetramer 4 * 50000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.2.2 additional information
-
 additional information pH-dependence of turnover-numbers Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.2.2 7
-
-
 Homo sapiens