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Literature summary extracted from

  • Appert, C.; Logermann, E.; Hahlbrock, K.; Schmid, J.; Amrhein, N.
    Structural and catalytic properties of the four phenylalanine ammonia-lyase isoenzymes from parsley (Petroselinum crispum Nym.) (1994), Eur. J. Biochem., 225, 491.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.24 fusion proteins with gluthatione S-transferase expressed in Escherichia coli Petroselinum crispum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.24 Cinnamic acid (E)-cinnamate, competitive Petroselinum crispum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.24 0.015
-
 L-Phe isoenzyme PAL-4 Petroselinum crispum
4.3.1.24 0.0169
-
 L-Phe isoenzyme PAL-2 Petroselinum crispum
4.3.1.24 0.0172
-
 L-Phe isoenzyme PAL-1 Petroselinum crispum
4.3.1.24 0.0245
-
 L-Phe isoenzyme PAL-3 Petroselinum crispum
4.3.1.24 2.3
-
 L-Tyr isoenzyme PAL-2 Petroselinum crispum
4.3.1.24 2.5
-
 L-Tyr isoenzyme PAL-1 Petroselinum crispum
4.3.1.24 2.6
-
 L-Tyr isoenzyme PAL-4 Petroselinum crispum
4.3.1.24 7.8
-
 L-Tyr isoenzyme PAL-3 Petroselinum crispum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.24 77828
-
 4 * 77828, calöculation from nucleotide sequence Petroselinum crispum
4.3.1.24 300000
-
 isoenzyme PAL 1, gel filtration Petroselinum crispum

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.24 Petroselinum crispum
-
 Nym., 4 isoenzymes: PAL 1, PAL 2, PAL 3, PAL 4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.24
-
 Petroselinum crispum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.24 L-Phe
-
 Petroselinum crispum (E)-cinnamate + NH3
-
 ?
4.3.1.24 L-tyrosine L-Tyr Petroselinum crispum p-coumarate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.24 tetramer 4 * 77828, calöculation from nucleotide sequence Petroselinum crispum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.24 58
-
-
 Petroselinum crispum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.24 0.3
-
 L-Tyr 30°C Petroselinum crispum
4.3.1.24 22
-
 L-Phe 30°C Petroselinum crispum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.24 8.5
-
-
 Petroselinum crispum