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Literature summary extracted from

  • Lim, H.W.; Sa, J.H.; Kim, T.S.; Park, S.S.; Lim, C.J.
    Purification and properties of phenylalanine ammonia-lyase from Chinese cabbage (1998), J. Biochem. Mol. Biol., 31, 31-36.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.1.24 caffeate strong activation Brassica rapa subsp. oleifera

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.24 Ca2+
-
 Brassica rapa subsp. oleifera
4.3.1.24 Cu2+
-
 Brassica rapa subsp. oleifera
4.3.1.24 Zn2+
-
 Brassica rapa subsp. oleifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.24 0.21
-
 L-Phe
-
 Brassica rapa subsp. oleifera

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.3.1.24 cytosol
-
 Brassica rapa subsp. oleifera 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.24 38000
-
 4 * 38000, SDS-PAGE Brassica rapa subsp. oleifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.24 additional information Brassica rapa subsp. oleifera first enzyme in phenylpropanoid biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.24 Brassica rapa subsp. oleifera
-
 ssp. napus var. pekinensis
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.24
-
 Brassica rapa subsp. oleifera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.24 L-Phe
-
 Brassica rapa subsp. oleifera (E)-cinnamate + NH3
-
 ?
4.3.1.24 additional information first enzyme in phenylpropanoid biosynthesis Brassica rapa subsp. oleifera ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.24 tetramer 4 * 38000, SDS-PAGE Brassica rapa subsp. oleifera

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.24 45
-
-
 Brassica rapa subsp. oleifera

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.3.1.24 15 60 15°C: about 25% of maximal activity, 60°C: no activity Brassica rapa subsp. oleifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.24 8 9
-
 Brassica rapa subsp. oleifera

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.1.24 7 10 pH 7: about 30% of maximal activity, pH 10: about 65% of maximal activity Brassica rapa subsp. oleifera