BRENDA - Enzyme Database show

Purification and functional characterization of glutamate-1-semialdehyde aminotransferase from Chlamydomonas reinhardtii

Jahn, D.; Chen, M.W.; S÷ll, D.; J. Biol. Chem. 266, 161-167 (1991)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.3.8
Aminooxyacetate
-
Chlamydomonas reinhardtii
5.4.3.8
gabaculine
-
Chlamydomonas reinhardtii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.3.8
43000
-
gel filtration, nondenaturing rate zonal sedimentation on glycerol gradients
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
Chlamydomonas reinhardtii
formation of 5-aminolevulinic acid, which is the first committed precursor of chlorophyll biosynthesis in the chloroplast
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.3.8
Chlamydomonas reinhardtii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.3.8
-
Chlamydomonas reinhardtii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.4.3.8
0.2
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
-
3436
Chlamydomonas reinhardtii
5-Amino-4-oxopentanoate
-
3436
Chlamydomonas reinhardtii
-
5.4.3.8
(S)-4-Amino-5-oxopentanoate
formation of 5-aminolevulinic acid, which is the first committed precursor of chlorophyll biosynthesis in the chloroplast
3436
Chlamydomonas reinhardtii
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
5.4.3.8
monomer
1 * 43000, SDS-PAGE
Chlamydomonas reinhardtii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.3.8
7.2
-
-
Chlamydomonas reinhardtii
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.4.3.8
6.5
8
pH 6.5: about 30% of maximal activity, pH 8.0: about 50% of maximal activity
Chlamydomonas reinhardtii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.3.8
pyridoxal 5'-phosphate
required
Chlamydomonas reinhardtii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.3.8
pyridoxal 5'-phosphate
required
Chlamydomonas reinhardtii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.3.8
Aminooxyacetate
-
Chlamydomonas reinhardtii
5.4.3.8
gabaculine
-
Chlamydomonas reinhardtii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.3.8
43000
-
gel filtration, nondenaturing rate zonal sedimentation on glycerol gradients
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
Chlamydomonas reinhardtii
formation of 5-aminolevulinic acid, which is the first committed precursor of chlorophyll biosynthesis in the chloroplast
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.3.8
-
Chlamydomonas reinhardtii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.4.3.8
0.2
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
-
3436
Chlamydomonas reinhardtii
5-Amino-4-oxopentanoate
-
3436
Chlamydomonas reinhardtii
-
5.4.3.8
(S)-4-Amino-5-oxopentanoate
formation of 5-aminolevulinic acid, which is the first committed precursor of chlorophyll biosynthesis in the chloroplast
3436
Chlamydomonas reinhardtii
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.3.8
monomer
1 * 43000, SDS-PAGE
Chlamydomonas reinhardtii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.3.8
7.2
-
-
Chlamydomonas reinhardtii
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.4.3.8
6.5
8
pH 6.5: about 30% of maximal activity, pH 8.0: about 50% of maximal activity
Chlamydomonas reinhardtii