BRENDA - Enzyme Database show

Gabaculine resistance of Synechococcus glutamate 1-semialdehyde aminotransferase

Smith, M.A.; Grimm, B.; Biochemistry 31, 4122-4127 (1992)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.3.8
wild-type enzyme and gabaculine-resistant mutant enzyme, overexpression in Escherichia coli
Synechococcus sp.
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.4.3.8
additional information
gabaculine-resistant mutant enzyme with a 3 times lower catalytic efficiency and impaired prototropic rearrangement and transaldimination
Synechococcus sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.3.8
gabaculine
wild-type enzyme is inhibited, gabaculine-resistant mutant enzyme is not inhibited
Synechococcus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.3.8
0.022
-
(S)-4-Amino-5-oxopentanoate
gabaculine-resistant mutant enzyme
Synechococcus sp.
5.4.3.8
0.046
-
(S)-4-Amino-5-oxopentanoate
wild-type enzyme
Synechococcus sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.3.8
Synechococcus sp.
-
wild-type strain and gabaculine-resistant strain
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.3.8
wild-type enzyme and gabaculine-resistant mutant enzyme
Synechococcus sp.
Reaction
EC Number
Reaction
Commentary
Organism
5.4.3.8
L-glutamate 1-semialdehyde = 5-aminolevulinate
wild-type enzyme and gabaculine-resistant mutant enzyme use a ping-pong bi-bi mechanism in which 4,5-diaminovalerate is a likely intermediate
Synechococcus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
-
3430
Synechococcus sp.
5-Amino-4-oxopentanoate
-
3430
Synechococcus sp.
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.3.8
0.077
-
(S)-4-Amino-5-oxopentanoate
gabaculine-resistant mutant enzyme
Synechococcus sp.
5.4.3.8
0.47
-
(S)-4-Amino-5-oxopentanoate
wild-type enzyme
Synechococcus sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.3.8
vitamin B6
wild-type enzyme and gabaculine-resistant mutant enzyme contain vitamin B6
Synechococcus sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.3.8
wild-type enzyme and gabaculine-resistant mutant enzyme, overexpression in Escherichia coli
Synechococcus sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.3.8
vitamin B6
wild-type enzyme and gabaculine-resistant mutant enzyme contain vitamin B6
Synechococcus sp.
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.4.3.8
additional information
gabaculine-resistant mutant enzyme with a 3 times lower catalytic efficiency and impaired prototropic rearrangement and transaldimination
Synechococcus sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.3.8
gabaculine
wild-type enzyme is inhibited, gabaculine-resistant mutant enzyme is not inhibited
Synechococcus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.3.8
0.022
-
(S)-4-Amino-5-oxopentanoate
gabaculine-resistant mutant enzyme
Synechococcus sp.
5.4.3.8
0.046
-
(S)-4-Amino-5-oxopentanoate
wild-type enzyme
Synechococcus sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.3.8
wild-type enzyme and gabaculine-resistant mutant enzyme
Synechococcus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.3.8
(S)-4-Amino-5-oxopentanoate
-
3430
Synechococcus sp.
5-Amino-4-oxopentanoate
-
3430
Synechococcus sp.
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.3.8
0.077
-
(S)-4-Amino-5-oxopentanoate
gabaculine-resistant mutant enzyme
Synechococcus sp.
5.4.3.8
0.47
-
(S)-4-Amino-5-oxopentanoate
wild-type enzyme
Synechococcus sp.