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Literature summary extracted from

  • Moran, F.; Nasuno, S.; Starr, M.P.
    Oligogalacturonide trans-eliminase of Erwinia carotovora (1968), Arch. Biochem. Biophys., 125, 734-741.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.6 1.6
-
 unsaturated digalacturonic acid
-
 Erwinia aroidea
4.2.2.6 5.3
-
 digalacturonic acid
-
 Erwinia aroidea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.6 additional information Erwinia aroidea two pathways are involved in metabolism of pectic substances: one route through galacturonic acid, involving galacturonic acid isomerase and the other through unsaturated digalacturonic acid, involving EC 4.2.2.5, with the formation of 4-deoxy-5-hexosulose uronic acid. Both pathways yield 2-keto-3-deoxy-D-gluconic acid as common product ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.6 Erwinia aroidea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.6
-
 Erwinia aroidea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.6 additional information
-
-
 Erwinia aroidea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.6 4-O-(4-deoxy-alpha-L-threo-hex-4-enopyranuronosyl)-beta-D-galactopyranuronic acid i.e. O-(4-deoxy-beta-L-5-threo-hexopyranos-4-enyluronic acid)-(1,4)-D-galacturonic acid Erwinia aroidea 4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid + D-galacturonic acid
-
 ?
4.2.2.6 digalacturonic acid
-
 Erwinia aroidea 4-deoxy-5-keto-D-fructuronic acid + ?
-
 ?
4.2.2.6 additional information two pathways are involved in metabolism of pectic substances: one route through galacturonic acid, involving galacturonic acid isomerase and the other through unsaturated digalacturonic acid, involving EC 4.2.2.5, with the formation of 4-deoxy-5-hexosulose uronic acid. Both pathways yield 2-keto-3-deoxy-D-gluconic acid as common product Erwinia aroidea ?
-
 ?
4.2.2.6 unsaturated digalacturonic acid
-
 Erwinia aroidea 4-deoxy-5-keto-D-fructuronic acid + ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.6 7.2
-
-
 Erwinia aroidea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.6 6.5 8 pH 6.5: about 45% of maximal activity, pH 8.0: about 55% of maximal activity Erwinia aroidea