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Literature summary extracted from

  • Linn, S.; Chan, T.; Lipeski, L.; Salyers, A.A.
    Isolation and characterization of two chondroitin lyases from Bacteroides thetaiotaomicron (1983), J. Bacteriol., 156, 859-866.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.21 phosphate activity in 20 mM phosphate buffer is less than half of the activity in 50 mM phosphate buffer, maximum activity for isoenzyme I in 50 mM, isoenzyme II in 50-100 mM phosphate Bacteroides thetaiotaomicron

General Stability

EC Number General Stability Organism
4.2.2.21 sensitive to lyophilization and freezing and thawing, both isoenzyme I and II Bacteroides thetaiotaomicron

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.21 heparin 50% inhibition at 0.050 mg/ml for enzyme I and 0.005 mg/ml for enzyme II Bacteroides thetaiotaomicron
4.2.2.21 N-desulfated heparin 50% inhibition at 0.150 mg/ml for enzyme I and 0.025 mg/ml for enzyme II Bacteroides thetaiotaomicron

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.21 104000
-
 x * 108000, isoenzyme I, x * 104000, isoenzyme II, SDS-PAGE Bacteroides thetaiotaomicron
4.2.2.21 108000
-
 x * 108000, isoenzyme I, x * 104000, isoenzyme II, SDS-PAGE Bacteroides thetaiotaomicron

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.21 Bacteroides thetaiotaomicron
-
 isoenzymes I and II
-

Storage Stability

EC Number Storage Stability Organism
4.2.2.21 4°C, two weeks, 70-80% residual activity, both isoenzyme I and II Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.21 chondroitin sulfate A
-
 Bacteroides thetaiotaomicron ?
-
 ?
4.2.2.21 chondroitin sulfate C
-
 Bacteroides thetaiotaomicron ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.21 ? x * 108000, isoenzyme I, x * 104000, isoenzyme II, SDS-PAGE Bacteroides thetaiotaomicron

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.21 7.1
-
 isoenzyme I Bacteroides thetaiotaomicron
4.2.2.21 7.6
-
 isoenzyme II Bacteroides thetaiotaomicron

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.21 Bacteroides thetaiotaomicron isoelectric focusing, isoenzyme I
-
 7.9
4.2.2.21 Bacteroides thetaiotaomicron isoelectric focusing, isoenzyme II
-
 8