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Literature summary extracted from

  • Pirrung, M.C.; Holmes, C.P.; Horowitz, D.M.; Nunn, D.S.
    Mechanism and stereochemistry of alpha,beta-dihydroxyacid dehydratase (1991), J. Am. Chem. Soc., 113, 1020-1025.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.9 0.33
-
(2R,3R)-2,3-dihydroxy-3-methylpentanoate
-
Spinacia oleracea
4.2.1.9 0.55
-
2,3-dihydroxybutanoate
-
Spinacia oleracea
4.2.1.9 0.71
-
(2R,3S)-2,3-dihydroxy-3-methylpentanoate
-
Spinacia oleracea
4.2.1.9 0.97
-
2,3-Dihydroxy-3-methylbutanoate
-
Spinacia oleracea
4.2.1.9 1.78
-
3-cyclopropyl-2,3-dihydroxybutanoate
-
Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.9 Spinacia oleracea
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.9 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O polar but concerted elimination mechanism which should proceed through an anti transition state Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.9 (2R,3R)-2,3-dihydroxy-3-methylpentanoate
-
Spinacia oleracea 3-methyl-2-oxopentanoate + H2O
-
?
4.2.1.9 (2R,3S)-2,3-dihydroxy-3-methylpentanoate
-
Spinacia oleracea 3-methyl-2-oxopentanoate + H2O
-
?
4.2.1.9 2,3-dihydroxy-3-methylbutanoate
-
Spinacia oleracea 2-oxoisovalerate + H2O
-
?
4.2.1.9 2,3-dihydroxy-3-methylpentanoate 2R,3S-2,3-dihydroxy-3-methylpentanoate, 2R,3R-2,3-dihydroxy-3-methylpentanoate Spinacia oleracea 3-methyl-2-oxopentanoate + H2O
-
?
4.2.1.9 2,3-dihydroxybutanoate
-
Spinacia oleracea ?
-
?
4.2.1.9 3-cyclopropyl-2,3-dihydroxybutanoate
-
Spinacia oleracea ?
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.9 4.6
-
2,3-dihydroxybutanoate
-
Spinacia oleracea
4.2.1.9 5.7
-
3-cyclopropyl-2,3-dihydroxybutanoate
-
Spinacia oleracea
4.2.1.9 8.3
-
(2R,3R)-2,3-dihydroxy-3-methylpentanoate
-
Spinacia oleracea
4.2.1.9 11.5
-
2,3-Dihydroxy-3-methylbutanoate
-
Spinacia oleracea
4.2.1.9 12.3
-
(2R,3R)-2,3-dihydroxy-3-methylpentanoate
-
Spinacia oleracea