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Literature summary extracted from

  • Baker, J.J.; van der Drift, C.; Stadtman, T.C.
    Purification and properties of beta-lysine mutase, a pyridoxal phosphate and B12 coenzyme dependent enzyme (1973), Biochemistry, 12, 1054-1063.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.3.3 2'-deoxyATP stimulates Acetoanaerobium sticklandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.3.3 Diethanolamine/HCl
-
 Acetoanaerobium sticklandii
5.4.3.3 hydrazine
-
 Acetoanaerobium sticklandii
5.4.3.3 Hydroxyadenylcobamide formed during isolation by degradation of enzyme-bound cobamide, strong inhibitor, tightly bound to the protein. Incubation with cobalamin, Mg2+, a mercaptan, and pyridoxal 5'-phosphate displaces the hydroxyadenylcobamide and markedly activates the enzyme Acetoanaerobium sticklandii
5.4.3.3 hydroxylamine
-
 Acetoanaerobium sticklandii
5.4.3.3 Isonicotinic acid hydrazide
-
 Acetoanaerobium sticklandii
5.4.3.3 additional information during catalysis under conditions where the mutase product 3,5-diaminohexanoate is not continuously removed there is extensive cleavage of cobamamide coenzyme to free 5'-deoxyadenosine and cobalamin with concomitant inactivation of the enzyme. This inactivation is prevented by the addition of sulfhydryl protein and ATP, oxygen accelerates inactivation Acetoanaerobium sticklandii
5.4.3.3 NaBH4
-
 Acetoanaerobium sticklandii
5.4.3.3 phenylhydrazine
-
 Acetoanaerobium sticklandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.3.3 K+ monovalent cation, e.g. K+ required Acetoanaerobium sticklandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.3.3 32000
-
 2 * 32000 + 2 * 52000, SDS-PAGE Acetoanaerobium sticklandii
5.4.3.3 52000
-
 2 * 32000 + 2 * 52000, SDS-PAGE Acetoanaerobium sticklandii
5.4.3.3 170000
-
 gel filtration Acetoanaerobium sticklandii

Organism

EC Number Organism UniProt Comment Textmining
5.4.3.3 Acetoanaerobium sticklandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.3.3
-
 Acetoanaerobium sticklandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.3.3 19.5
-
 unit defined as the amounts which allows the formation of 0.001 mM NADPH per min in a coupled assay system Acetoanaerobium sticklandii

Storage Stability

EC Number Storage Stability Organism
5.4.3.3 -22°C, stable for at least 3 months. After 6 months, with repeated freezing and thawing, two-third of the original activity remains Acetoanaerobium sticklandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.3.3 3,6-Diaminohexanoate
-
 Acetoanaerobium sticklandii 3,5-Diaminohexanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.3.3 tetramer 2 * 32000 + 2 * 52000, SDS-PAGE Acetoanaerobium sticklandii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.3.3 7.8
-
 highest activity in triethanolamine/HCl buffer Acetoanaerobium sticklandii

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.3.3 Cobalamin dependent on Acetoanaerobium sticklandii
5.4.3.3 pyridoxal 5'-phosphate absolutely dependent on, Km: 0.00022 mM Acetoanaerobium sticklandii