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Literature summary extracted from

  • Smiley, J.D.; Ashwell, G.
    Uronic acid metabolism in bacteria. III. Purification and properties of D-altronic acid and D-mannonic acid dehydrases in Escherichia coli (1960), J. Biol. Chem., 235, 1571-1575.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.7 Cys increases activity Escherichia coli

General Stability

EC Number General Stability Organism
4.2.1.7 addition of high concentrations, 0.1 M, of Cys or mercaptoethanol and maintenance in pH range pH 7.0-8.0 stabilizes throughout purification Escherichia coli
4.2.1.7 freezing, agitation or stirring in the presence of air or dialysis at pH levels below pH 7.0 inactivates Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.7 EDTA
-
 Escherichia coli
4.2.1.7 PCMB
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.7 additional information
-
 additional information
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.7 Fe2+ optimal activation at 0.2 mM or greater Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.7 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.7
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
4.2.1.7 0-5°C, loss of activity after 2-3 weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.7 D-altronate
-
 Escherichia coli 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.7 7 8.2
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.7 7
-
 dialysis below pH 7 inactivates Escherichia coli