Literature summary extracted from
Blickling, S.; Beisel, H.G.; Bozic, D.; Knaeblein, J.; Laber, B.; Huber, R.
Structure of dihydrodipicolinate synthase of Nicotiana sylvestris reveals novel quaternary structure (1997), J. Mol. Biol., 274, 608-621.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.3.3.7 |
crystal structure at 2.8 A resolution |
Nicotiana sylvestris |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.3.3.7 |
L-lysine |
discussion of plant inhibition versus bacterial inhibition |
Nicotiana sylvestris |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.3.3.7 |
Nicotiana sylvestris |
- |
tobacco |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
4.3.3.7 |
pyruvate + L-aspartate-4-semialdehyde = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O |
the reaction is initiated by an nucleophilic attack of the epsilon-amino group of active site lysine to the C2 atom of pyruvate with subsequent imine formation. Tautomerization of the imine to the enamine creates the nucleophile necessary for the attack of the aldehyde group of L-aspartate semialdehyde. Cyclization of the substrate coupled with transamination leads to stepwise detachment from the active site |
Nicotiana sylvestris |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.3.3.7 |
L-aspartate-4-semialdehyde + pyruvate |
- |
Nicotiana sylvestris |
dihydrodipicolinate + H2O |
(4S)-4-hydroxy-2,3,4,5-terahydro-(2S)-dipicolinic acid is the initial but instable product |
? |
|