BRENDA - Enzyme Database

Partial purification and some properties of pyruvate-aspartic semialdehyde condensing enzyme from sporulating Bacillus subtilis

Yamakura, F.; Ikeda, Y.; Kimura, K.; Sasakawa, T.; J. Biochem. 76, 611-621 (1974)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.3.3.7
isocitric acid
activation
Bacillus subtilis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
DL-diaminopimelic acid
-
Bacillus subtilis
4.3.3.7
glyoxylic acid
-
Bacillus subtilis
4.3.3.7
L-lysine
no inhibition
Bacillus subtilis
4.3.3.7
additional information
not inhibitory: ATP, ADP, AMP, citric acid, L-malic acid, 2-oxoglutaric acid, fumaric acid, succinic acid, L-glutamic acid, L-threonine
Bacillus subtilis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
1.07
-
pyruvate
-
Bacillus subtilis
4.3.3.7
3.13
-
L-aspartate-4-semialdehyde
-
Bacillus subtilis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.3.7
additional information
no effect: Cd2+, Mg2+, Mn2+, Zn2+
Bacillus subtilis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
124000
-
gel filtration
Bacillus subtilis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Bacillus subtilis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
partial
Bacillus subtilis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.3.7
458
-
-
Bacillus subtilis
Storage Stability
EC Number
Storage Stability
Organism
4.3.3.7
-20°C
Bacillus subtilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33906
Bacillus subtilis
dihydrodipicolinate + H2O
-
33906
Bacillus subtilis
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.3.7
9.5
-
-
Bacillus subtilis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.3.3.7
isocitric acid
activation
Bacillus subtilis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.3.7
DL-diaminopimelic acid
-
Bacillus subtilis
4.3.3.7
glyoxylic acid
-
Bacillus subtilis
4.3.3.7
L-lysine
no inhibition
Bacillus subtilis
4.3.3.7
additional information
not inhibitory: ATP, ADP, AMP, citric acid, L-malic acid, 2-oxoglutaric acid, fumaric acid, succinic acid, L-glutamic acid, L-threonine
Bacillus subtilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.3.7
1.07
-
pyruvate
-
Bacillus subtilis
4.3.3.7
3.13
-
L-aspartate-4-semialdehyde
-
Bacillus subtilis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.3.7
additional information
no effect: Cd2+, Mg2+, Mn2+, Zn2+
Bacillus subtilis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
124000
-
gel filtration
Bacillus subtilis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
partial
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.3.7
458
-
-
Bacillus subtilis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.3.3.7
-20°C
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate-4-semialdehyde + pyruvate
-
33906
Bacillus subtilis
dihydrodipicolinate + H2O
-
33906
Bacillus subtilis
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.3.7
9.5
-
-
Bacillus subtilis