BRENDA - Enzyme Database

Parallel partial purification of cytoplasmic and mitochondrial aconitate hydratases from rat liver

Guarriero-Bobyleva, V.; Volpi-Becchi, M.A.; Masini, A.; Eur. J. Biochem. 34, 455-458 (1973)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
0.08
-
isocitrate
cytoplasmic isoenzyme
Rattus norvegicus
4.2.1.3
0.12
-
isocitrate
mitochondrial enzyme
Rattus norvegicus
4.2.1.3
0.41
-
citrate
cytoplasmic isoenzyme
Rattus norvegicus
4.2.1.3
0.48
-
citrate
mitochondrial isoenzyme
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.3
cytoplasm
-
Rattus norvegicus
5737
-
4.2.1.3
mitochondrion
-
Rattus norvegicus
5739
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.3
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.3
partial
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.3
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.3
1.11
-
cytoplasmic isoenzyme
Rattus norvegicus
4.2.1.3
3.4
-
mitochondrial isoenzyme
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
citrate
-
33797
Rattus norvegicus
isocitrate
-
-
-
?
4.2.1.3
isocitrate
-
33797
Rattus norvegicus
citrate
-
-
-
?
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
0.08
-
isocitrate
cytoplasmic isoenzyme
Rattus norvegicus
4.2.1.3
0.12
-
isocitrate
mitochondrial enzyme
Rattus norvegicus
4.2.1.3
0.41
-
citrate
cytoplasmic isoenzyme
Rattus norvegicus
4.2.1.3
0.48
-
citrate
mitochondrial isoenzyme
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.3
cytoplasm
-
Rattus norvegicus
5737
-
4.2.1.3
mitochondrion
-
Rattus norvegicus
5739
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
partial
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.3
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.3
1.11
-
cytoplasmic isoenzyme
Rattus norvegicus
4.2.1.3
3.4
-
mitochondrial isoenzyme
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
citrate
-
33797
Rattus norvegicus
isocitrate
-
-
-
?
4.2.1.3
isocitrate
-
33797
Rattus norvegicus
citrate
-
-
-
?