BRENDA - Enzyme Database

Studies on two isozymes of aconitase from Bacillus cereus T. III. Enzymatic properties

Agrawal, P.K.; Garg, G.K.; Gollakota, K.G.; Biochem. Biophys. Res. Commun. 70, 987-996 (1976)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.3
ethyl picolinate
isoenzyme is inhibited, isoenzyme I is less or not sensitive
Bacillus cereus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
0.7
-
isocitrate
isoenzyme II
Bacillus cereus
4.2.1.3
1.4
-
citrate
isoenzyme II
Bacillus cereus
4.2.1.3
3.33
-
isocitrate
isoenzyme I
Bacillus cereus
4.2.1.3
10
-
citrate
isoenzyme I
Bacillus cereus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.3
75000
80000
isoenzyme II, gel filtration
Bacillus cereus
4.2.1.3
150000
160000
isoenzyme I, gel filtration
Bacillus cereus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.3
Bacillus cereus
-
early aconitase (isoenzyme I) is present in a culture of 5 h age, late aconitase (isoenzyme II) is present in 12 h age
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.3
-
Bacillus cereus
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.3
30
35
isoenzyme I
Bacillus cereus
4.2.1.3
30
-
isoenzyme II
Bacillus cereus
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4.2.1.3
20
40
about 60% of maximal activity at 20C and at 40C, isoenzyme I. 20C: about 40% of maximal activity, 40C: about 20% of maximal activity, isoenzyme II
Bacillus cereus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
7.5
8
isoenzyme I
Bacillus cereus
4.2.1.3
7.5
-
isoenzyme II
Bacillus cereus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.3
6
9
pH 6: about 55% of maximal activity, pH 9: about 40% of maximal activity, isoenzyme I. pH 6: about 25% of maximal activity, pH 9: about 75% of maximal activity isoenzyme II
Bacillus cereus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.3
ethyl picolinate
isoenzyme is inhibited, isoenzyme I is less or not sensitive
Bacillus cereus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
0.7
-
isocitrate
isoenzyme II
Bacillus cereus
4.2.1.3
1.4
-
citrate
isoenzyme II
Bacillus cereus
4.2.1.3
3.33
-
isocitrate
isoenzyme I
Bacillus cereus
4.2.1.3
10
-
citrate
isoenzyme I
Bacillus cereus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.3
75000
80000
isoenzyme II, gel filtration
Bacillus cereus
4.2.1.3
150000
160000
isoenzyme I, gel filtration
Bacillus cereus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
-
Bacillus cereus
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.3
30
35
isoenzyme I
Bacillus cereus
4.2.1.3
30
-
isoenzyme II
Bacillus cereus
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4.2.1.3
20
40
about 60% of maximal activity at 20C and at 40C, isoenzyme I. 20C: about 40% of maximal activity, 40C: about 20% of maximal activity, isoenzyme II
Bacillus cereus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
7.5
8
isoenzyme I
Bacillus cereus
4.2.1.3
7.5
-
isoenzyme II
Bacillus cereus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.3
6
9
pH 6: about 55% of maximal activity, pH 9: about 40% of maximal activity, isoenzyme I. pH 6: about 25% of maximal activity, pH 9: about 75% of maximal activity isoenzyme II
Bacillus cereus