BRENDA - Enzyme Database

Enzymatic properties of enolase from fish muscle

Nakagawa, T.; Nagayama, F.; Comp. Biochem. Physiol. B 98, 355-359 (1991)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
phosphate
competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate
Cyprinus carpio
4.2.1.11
phosphate
competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate
Oryctolagus cuniculus
4.2.1.11
phosphate
competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate
Pagrus major
4.2.1.11
phosphate
competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate
Scomber japonicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.038
-
2-phosphoglycerate
-
Scomber japonicus
4.2.1.11
0.05
-
2-phosphoglycerate
-
Pagrus major
4.2.1.11
0.061
-
2-phosphoglycerate
-
Oryctolagus cuniculus
4.2.1.11
0.069
-
2-phosphoglycerate
-
Cyprinus carpio
4.2.1.11
0.11
-
phosphoenolpyruvate
-
Scomber japonicus
4.2.1.11
0.2
-
phosphoenolpyruvate
-
Cyprinus carpio
4.2.1.11
0.2
-
phosphoenolpyruvate
-
Pagrus major
4.2.1.11
0.25
-
phosphoenolpyruvate
-
Oryctolagus cuniculus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
absolute requirement for divalent metal ions, Mg2+ is most effective; Km: 0.67 mM
Cyprinus carpio
4.2.1.11
Mg2+
absolute requirement for divalent metal ions, Mg2+ is most effective; Km: 0.69 mM
Oryctolagus cuniculus
4.2.1.11
Mg2+
absolute requirement for divalent metal ions, Mg2+ is most effective; Km: 0.7 mM
Pagrus major
4.2.1.11
Mg2+
absolute requirement for divalent metal ions, Mg2+ is most effective; Km: 0.73 mM
Scomber japonicus
4.2.1.11
Mn2+
can partially replace Mg2+ in activation
Cyprinus carpio
4.2.1.11
Mn2+
can partially replace Mg2+ in activation
Oryctolagus cuniculus
4.2.1.11
Mn2+
can partially replace Mg2+ in activation
Pagrus major
4.2.1.11
Mn2+
can partially replace Mg2+ in activation
Scomber japonicus
4.2.1.11
Zn2+
can partially replace Mg2+ in activation
Cyprinus carpio
4.2.1.11
Zn2+
can partially replace Mg2+ in activation
Oryctolagus cuniculus
4.2.1.11
Zn2+
can partially replace Mg2+ in activation
Pagrus major
4.2.1.11
Zn2+
can partially replace Mg2+ in activation
Scomber japonicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
48000
-
2 * 48000, SDS-PAGE
Oryctolagus cuniculus
4.2.1.11
50000
-
2 * 50000, SDS-PAGE
Scomber japonicus
4.2.1.11
51000
-
2 * 51000, SDS-PAGE
Cyprinus carpio
4.2.1.11
52000
-
2 * 52000, SDS-PAGE
Pagrus major
4.2.1.11
92000
-
gel filtration
Oryctolagus cuniculus
4.2.1.11
93000
-
gel filtration
Scomber japonicus
4.2.1.11
94000
-
gel filtration
Cyprinus carpio
4.2.1.11
95500
-
gel filtration
Pagrus major
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Cyprinus carpio
-
-
-
4.2.1.11
Oryctolagus cuniculus
-
-
-
4.2.1.11
Pagrus major
-
-
-
4.2.1.11
Scomber japonicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
muscle
-
Cyprinus carpio
-
4.2.1.11
muscle
-
Pagrus major
-
4.2.1.11
muscle
-
Scomber japonicus
-
4.2.1.11
muscle
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
56.1
-
-
Scomber japonicus
4.2.1.11
61.7
-
-
Scomber japonicus
4.2.1.11
76.2
-
-
Pagrus major
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33631
Oryctolagus cuniculus
phosphoenolpyruvate
-
33631
Oryctolagus cuniculus
r
4.2.1.11
2-phospho-D-glycerate
-
33631
Pagrus major
phosphoenolpyruvate
-
33631
Pagrus major
r
4.2.1.11
2-phospho-D-glycerate
-
33631
Scomber japonicus
phosphoenolpyruvate
-
33631
Scomber japonicus
r
4.2.1.11
2-phospho-D-glycerate
-
33631
Cyprinus carpio
phosphoenolpyruvate
-
33631
Cyprinus carpio
r
4.2.1.11
phosphoenolpyruvate
-
33631
Oryctolagus cuniculus
2-phospho-D-glycerate
-
33631
Oryctolagus cuniculus
r
4.2.1.11
phosphoenolpyruvate
-
33631
Pagrus major
2-phospho-D-glycerate
-
33631
Pagrus major
r
4.2.1.11
phosphoenolpyruvate
-
33631
Scomber japonicus
2-phospho-D-glycerate
-
33631
Scomber japonicus
r
4.2.1.11
phosphoenolpyruvate
-
33631
Cyprinus carpio
2-phospho-D-glycerate
-
33631
Cyprinus carpio
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 51000, SDS-PAGE
Cyprinus carpio
4.2.1.11
dimer
2 * 48000, SDS-PAGE
Oryctolagus cuniculus
4.2.1.11
dimer
2 * 52000, SDS-PAGE
Pagrus major
4.2.1.11
dimer
2 * 50000, SDS-PAGE
Scomber japonicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.6
-
formation of 2-phosphoglycerate
Cyprinus carpio
4.2.1.11
6.6
-
formation of 2-phosphoglycerate
Scomber japonicus
4.2.1.11
6.9
-
formation of phosphoenolpyruvate
Cyprinus carpio
4.2.1.11
6.9
-
formation of phosphoenolpyruvate
Oryctolagus cuniculus
4.2.1.11
7
-
-
Oryctolagus cuniculus
4.2.1.11
7.1
-
formation of 2-phosphoglycerate
Pagrus major
4.2.1.11
7.1
-
formation of phosphoenolpyruvate
Scomber japonicus
4.2.1.11
7.3
-
formation of phosphoenolpyruvate
Pagrus major
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
phosphate
competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate
Cyprinus carpio
4.2.1.11
phosphate
competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate
Oryctolagus cuniculus
4.2.1.11
phosphate
competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate
Pagrus major
4.2.1.11
phosphate
competitive inhibition at 2-4 mM phosphate with respect to 2-phosphoglycerate becomes noncompetitive in presence of 20-40 mM phosphate
Scomber japonicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.038
-
2-phosphoglycerate
-
Scomber japonicus
4.2.1.11
0.05
-
2-phosphoglycerate
-
Pagrus major
4.2.1.11
0.061
-
2-phosphoglycerate
-
Oryctolagus cuniculus
4.2.1.11
0.069
-
2-phosphoglycerate
-
Cyprinus carpio
4.2.1.11
0.11
-
phosphoenolpyruvate
-
Scomber japonicus
4.2.1.11
0.2
-
phosphoenolpyruvate
-
Cyprinus carpio
4.2.1.11
0.2
-
phosphoenolpyruvate
-
Pagrus major
4.2.1.11
0.25
-
phosphoenolpyruvate
-
Oryctolagus cuniculus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
absolute requirement for divalent metal ions, Mg2+ is most effective; Km: 0.67 mM
Cyprinus carpio
4.2.1.11
Mg2+
absolute requirement for divalent metal ions, Mg2+ is most effective; Km: 0.69 mM
Oryctolagus cuniculus
4.2.1.11
Mg2+
absolute requirement for divalent metal ions, Mg2+ is most effective; Km: 0.7 mM
Pagrus major
4.2.1.11
Mg2+
absolute requirement for divalent metal ions, Mg2+ is most effective; Km: 0.73 mM
Scomber japonicus
4.2.1.11
Mn2+
can partially replace Mg2+ in activation
Cyprinus carpio
4.2.1.11
Mn2+
can partially replace Mg2+ in activation
Oryctolagus cuniculus
4.2.1.11
Mn2+
can partially replace Mg2+ in activation
Pagrus major
4.2.1.11
Mn2+
can partially replace Mg2+ in activation
Scomber japonicus
4.2.1.11
Zn2+
can partially replace Mg2+ in activation
Cyprinus carpio
4.2.1.11
Zn2+
can partially replace Mg2+ in activation
Oryctolagus cuniculus
4.2.1.11
Zn2+
can partially replace Mg2+ in activation
Pagrus major
4.2.1.11
Zn2+
can partially replace Mg2+ in activation
Scomber japonicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
48000
-
2 * 48000, SDS-PAGE
Oryctolagus cuniculus
4.2.1.11
50000
-
2 * 50000, SDS-PAGE
Scomber japonicus
4.2.1.11
51000
-
2 * 51000, SDS-PAGE
Cyprinus carpio
4.2.1.11
52000
-
2 * 52000, SDS-PAGE
Pagrus major
4.2.1.11
92000
-
gel filtration
Oryctolagus cuniculus
4.2.1.11
93000
-
gel filtration
Scomber japonicus
4.2.1.11
94000
-
gel filtration
Cyprinus carpio
4.2.1.11
95500
-
gel filtration
Pagrus major
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
muscle
-
Cyprinus carpio
-
4.2.1.11
muscle
-
Pagrus major
-
4.2.1.11
muscle
-
Scomber japonicus
-
4.2.1.11
muscle
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
56.1
-
-
Scomber japonicus
4.2.1.11
61.7
-
-
Scomber japonicus
4.2.1.11
76.2
-
-
Pagrus major
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
33631
Oryctolagus cuniculus
phosphoenolpyruvate
-
33631
Oryctolagus cuniculus
r
4.2.1.11
2-phospho-D-glycerate
-
33631
Pagrus major
phosphoenolpyruvate
-
33631
Pagrus major
r
4.2.1.11
2-phospho-D-glycerate
-
33631
Scomber japonicus
phosphoenolpyruvate
-
33631
Scomber japonicus
r
4.2.1.11
2-phospho-D-glycerate
-
33631
Cyprinus carpio
phosphoenolpyruvate
-
33631
Cyprinus carpio
r
4.2.1.11
phosphoenolpyruvate
-
33631
Oryctolagus cuniculus
2-phospho-D-glycerate
-
33631
Oryctolagus cuniculus
r
4.2.1.11
phosphoenolpyruvate
-
33631
Pagrus major
2-phospho-D-glycerate
-
33631
Pagrus major
r
4.2.1.11
phosphoenolpyruvate
-
33631
Scomber japonicus
2-phospho-D-glycerate
-
33631
Scomber japonicus
r
4.2.1.11
phosphoenolpyruvate
-
33631
Cyprinus carpio
2-phospho-D-glycerate
-
33631
Cyprinus carpio
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 51000, SDS-PAGE
Cyprinus carpio
4.2.1.11
dimer
2 * 48000, SDS-PAGE
Oryctolagus cuniculus
4.2.1.11
dimer
2 * 52000, SDS-PAGE
Pagrus major
4.2.1.11
dimer
2 * 50000, SDS-PAGE
Scomber japonicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.6
-
formation of 2-phosphoglycerate
Cyprinus carpio
4.2.1.11
6.6
-
formation of 2-phosphoglycerate
Scomber japonicus
4.2.1.11
6.9
-
formation of phosphoenolpyruvate
Cyprinus carpio
4.2.1.11
6.9
-
formation of phosphoenolpyruvate
Oryctolagus cuniculus
4.2.1.11
7
-
-
Oryctolagus cuniculus
4.2.1.11
7.1
-
formation of 2-phosphoglycerate
Pagrus major
4.2.1.11
7.1
-
formation of phosphoenolpyruvate
Scomber japonicus
4.2.1.11
7.3
-
formation of phosphoenolpyruvate
Pagrus major