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Literature summary extracted from

  • Demidkina, T.V.; Myagkikh, I.V.; Azhayev, A.V.
    Transamination catalysed by tyrosine phenol-lyase from Citrobacter intermedius (1987), Eur. J. Biochem., 170, 311-316.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.99.2 L-Ala competitive Citrobacter intermedius
4.1.99.2 L-m-Tyr competitive Citrobacter intermedius
4.1.99.2 L-Phe competitive Citrobacter intermedius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.99.2 0.24
-
 Tyr beta-elimination Citrobacter intermedius
4.1.99.2 0.94
-
 L-m-Tyr transamination Citrobacter intermedius
4.1.99.2 4.15
-
 Phe beta-elimination Citrobacter intermedius
4.1.99.2 6.1
-
 Ala beta-elimination Citrobacter intermedius
4.1.99.2 34
-
 Ser beta-elimination Citrobacter intermedius
4.1.99.2 51
-
 Ser transamination Citrobacter intermedius

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.2 Citrobacter intermedius
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.2 L-Ala + pyridoxal phosphate
-
 Citrobacter intermedius pyridoxamine phosphate + keto acid
-
 ir
4.1.99.2 L-m-Tyr + pyridoxal phosphate
-
 Citrobacter intermedius pyridoxamine phosphate + keto acid
-
 ir
4.1.99.2 L-Phe + pyridoxal phosphate
-
 Citrobacter intermedius pyridoxamine phosphate + keto acid
-
 ir
4.1.99.2 L-Ser + pyridoxal phosphate
-
 Citrobacter intermedius pyridoxamine phosphate + keto acid
-
 ir
4.1.99.2 Ser + H2O
-
 Citrobacter intermedius ? + pyruvate + NH3
-
 ?
4.1.99.2 Tyr + H2O L-Tyr Citrobacter intermedius phenol + pyruvate + NH3
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.99.2 0.0008
-
 Phe beta-elimination Citrobacter intermedius
4.1.99.2 0.001
-
 Ser transamination Citrobacter intermedius
4.1.99.2 0.00153
-
 Ala beta-elimination Citrobacter intermedius
4.1.99.2 0.00268
-
 L-m-Tyr transamination Citrobacter intermedius
4.1.99.2 0.012
-
 Ser beta-elimination Citrobacter intermedius
4.1.99.2 0.2
-
 Tyr beta-elimination Citrobacter intermedius