Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nees, S.; Schauer, R.; Mayer, F.
    Purification and characterization of N-acetylneuraminate lyase from Clostridium perfringens (1976), Hoppe-Seyler's Z. Physiol. Chem., 357, 839-853.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.3 Cu2+ 70% residual activity at 1 mM Clostridium perfringens
4.1.3.3 Fe2+ 30% residual activity at 1 mM Clostridium perfringens
4.1.3.3 heavy metal ions
-
 Clostridium perfringens
4.1.3.3 Hg2+ 0% residual activity at 1 mM Clostridium perfringens
4.1.3.3 pyruvate elimination Clostridium perfringens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.3 additional information
-
 additional information with substrate N-acetylneuraminic acid increasing Km with increasing pH Clostridium perfringens
4.1.3.3 2.8
-
 N-acetylneuraminic acid
-
 Clostridium perfringens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.3.3 cytoplasm
-
 Clostridium perfringens 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.3 additional information
-
 Clostridium perfringens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.3 48000
-
 2 * 48000, SDS-PAGE Clostridium perfringens
4.1.3.3 97000
-
 gel filtration Clostridium perfringens
4.1.3.3 100000
-
 ultracentrifugation Clostridium perfringens

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.3 Clostridium perfringens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.3
-
 Clostridium perfringens

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.3.3 aceneuramate = N-acetyl-D-mannosamine + pyruvate mechanism Clostridium perfringens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.3 N-Acetyl-D-mannosamine + pyruvate
-
 Clostridium perfringens N-Acetylneuraminic acid
-
 r
4.1.3.3 N-Acetylneuraminic acid
-
 Clostridium perfringens N-Acetyl-D-mannosamine + pyruvate
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.3.3 dimer 2 * 48000, SDS-PAGE Clostridium perfringens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.3 37
-
 enzyme assay at Clostridium perfringens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.3 16.7
-
 N-acetylneuraminic acid
-
 Clostridium perfringens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.3 7.2
-
 elimination Clostridium perfringens