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Literature summary extracted from

  • Hacking, A.J.; Quayle, J.R.
    Purification and properties of malyl-coenzyme A lyase from Pseudomonas AM1 (1974), Biochem. J., 139, 399-405.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.24 (2R)-4-malyl-CoA
-
 Methylorubrum extorquens
4.1.3.24 iodoacetate
-
 Methylorubrum extorquens
4.1.3.24 NEM
-
 Methylorubrum extorquens
4.1.3.24 PCMB
-
 Methylorubrum extorquens
4.1.3.24 Tris
-
 Methylorubrum extorquens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.24 0.015
-
 acetyl-CoA
-
 Methylorubrum extorquens
4.1.3.24 0.066
-
 (3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
 Methylorubrum extorquens
4.1.3.24 1.7
-
 glyoxylate
-
 Methylorubrum extorquens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.24 Co2+ divalent cation required, Mg2+ or Co2+ is most effective Methylorubrum extorquens
4.1.3.24 Mg2+ Km: 1.2 mM Methylorubrum extorquens
4.1.3.24 Mg2+ divalent cation required, Mg2+ or Co2+ is most effective Methylorubrum extorquens
4.1.3.24 Mn2+ divalent cation required, 88% of the activation with Mg2+ and Co2+ Methylorubrum extorquens
4.1.3.24 Zn2+ divalent cation required, 41% of the activation with Mg2+ and Co2+ Methylorubrum extorquens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.24 190000
-
 meniscus depletion method Methylorubrum extorquens

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.24 Methylorubrum extorquens
-
-
-
4.1.3.24 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.24
-
 Methylorubrum extorquens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.24 additional information
-
-
 Pseudomonas sp.
4.1.3.24 additional information
-
-
 Methylorubrum extorquens

Storage Stability

EC Number Storage Stability Organism
4.1.3.24 -15°C, stable for several months Methylorubrum extorquens
4.1.3.24 0-4°C, 2-3 weeks, stable Methylorubrum extorquens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.24 (3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
 Pseudomonas sp. acetyl-CoA + glyoxylate
-
 r
4.1.3.24 (3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
 Methylorubrum extorquens acetyl-CoA + glyoxylate
-
 r
4.1.3.24 acetyl-CoA + glyoxylate
-
 Pseudomonas sp. (3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
 r
4.1.3.24 acetyl-CoA + glyoxylate
-
 Methylorubrum extorquens (3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.24 55
-
 irreversible loss of activity Methylorubrum extorquens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.24 7.8
-
-
 Methylorubrum extorquens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.3.24 6.5 10 pH 6.5: about 55% of maximal activity, pH 10.0: about 45% of maximal activity Methylorubrum extorquens