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Literature summary extracted from

  • Giachetti, E.G.; Pinzauti, G.; Bonaccorsi, R.; Vincenzini, M.T.; Vanni, P.
    Isocitrate lyase from higher plants (1987), Phytochemistry, 26, 2439-2446.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.3.1 additional information no evidence that isocitrate lyase contains coenzyme Lupinus sp.
4.1.3.1 additional information no evidence that isocitrate lyase contains coenzyme Ricinus communis
4.1.3.1 additional information no evidence that isocitrate lyase contains coenzyme Cucumis sativus
4.1.3.1 additional information no evidence that isocitrate lyase contains coenzyme Helianthus annuus
4.1.3.1 additional information no evidence that isocitrate lyase contains coenzyme Linum usitatissimum
4.1.3.1 additional information no evidence that isocitrate lyase contains coenzyme Citrullus lanatus subsp. vulgaris
4.1.3.1 additional information no evidence that isocitrate lyase contains coenzyme Pinus densiflora
4.1.3.1 additional information no evidence that isocitrate lyase contains coenzyme Pinus pinea

General Stability

EC Number General Stability Organism
4.1.3.1 most isocitrases very unstable, bacterial enzyme more stable than plant enzyme Helianthus annuus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 citrate
-
 Helianthus annuus
4.1.3.1 citrate
-
 Ricinus communis
4.1.3.1 glycolate
-
 Helianthus annuus
4.1.3.1 glycolate
-
 Lupinus sp.
4.1.3.1 glycolate
-
 Pinus pinea
4.1.3.1 glyoxylate
-
 Linum usitatissimum
4.1.3.1 glyoxylate
-
 Lupinus sp.
4.1.3.1 glyoxylate
-
 Pinus pinea
4.1.3.1 malate
-
 Lupinus sp.
4.1.3.1 malate
-
 Ricinus communis
4.1.3.1 Maleate
-
 Pinus densiflora
4.1.3.1 Maleate
-
 Ricinus communis
4.1.3.1 malonate
-
 Citrullus lanatus subsp. vulgaris
4.1.3.1 malonate
-
 Linum usitatissimum
4.1.3.1 malonate
-
 Lupinus sp.
4.1.3.1 malonate
-
 Ricinus communis
4.1.3.1 methylenesuccinate
-
 Citrullus lanatus subsp. vulgaris
4.1.3.1 methylenesuccinate
-
 Linum usitatissimum
4.1.3.1 methylenesuccinate
-
 Lupinus sp.
4.1.3.1 methylenesuccinate
-
 Pinus densiflora
4.1.3.1 methylenesuccinate
-
 Pinus pinea
4.1.3.1 oxalate
-
 Citrullus lanatus subsp. vulgaris
4.1.3.1 oxalate
-
 Helianthus annuus
4.1.3.1 oxalate
-
 Lupinus sp.
4.1.3.1 oxalate
-
 Pinus densiflora
4.1.3.1 oxalate
-
 Pinus pinea
4.1.3.1 phosphoenolpyruvate
-
 Linum usitatissimum
4.1.3.1 phosphoenolpyruvate
-
 Lupinus sp.
4.1.3.1 phosphoenolpyruvate
-
 Pinus densiflora
4.1.3.1 succinate
-
 Helianthus annuus
4.1.3.1 succinate
-
 Linum usitatissimum
4.1.3.1 succinate
-
 Lupinus sp.
4.1.3.1 succinate
-
 Pinus densiflora
4.1.3.1 succinate
-
 Pinus pinea
4.1.3.1 succinate
-
 Ricinus communis
4.1.3.1 Tartrate
-
 Citrullus lanatus subsp. vulgaris
4.1.3.1 Tartrate
-
 Linum usitatissimum
4.1.3.1 Tartrate
-
 Lupinus sp.
4.1.3.1 Tartrate
-
 Pinus densiflora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.032
-
 isocitrate phosphate, pH 6.0 Helianthus annuus
4.1.3.1 0.033
-
 Ds-isocitrate HEPES, pH 7.0 Pinus pinea
4.1.3.1 0.035
-
 isocitrate imidazole, pH 7.0 Lupinus sp.
4.1.3.1 0.039
-
 isocitrate MOPS, pH 6.8 Cucumis sativus
4.1.3.1 0.1
-
 threo-Ds-isocitrate Hepes, pH 7.4 Cucumis sativus
4.1.3.1 0.11
-
 isocitrate TRA, pH 7.0 Pinus pinea
4.1.3.1 0.25
-
 isocitrate MOPS, pH 7.5 Citrullus lanatus subsp. vulgaris
4.1.3.1 0.29
-
 isocitrate phosphate, pH 7.5 Linum usitatissimum
4.1.3.1 0.3
-
 isocitrate phosphate, pH 7.0 Ricinus communis
4.1.3.1 0.66
-
 isocitrate Tris, pH 7.6 Pinus densiflora
4.1.3.1 0.67
-
 isocitrate phosphate, pH 7.5 Helianthus annuus
4.1.3.1 1
-
 isocitrate phosphate, pH 6.8 Ricinus communis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.3.1 glyoxysome matrix Cucumis sativus 9514
-
4.1.3.1 glyoxysome matrix Helianthus annuus 9514
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Mg2+
-
 Pinus densiflora
4.1.3.1 Mg2+ maximal activity at 5-6 mM Cucumis sativus
4.1.3.1 Mg2+ maximal activity at 3.7 mM Linum usitatissimum
4.1.3.1 Mg2+ maximal activity at 5 mM Lupinus sp.
4.1.3.1 Mg2+ maximal activity at 6 mM Pinus pinea
4.1.3.1 Mg2+ increase of activity Lupinus sp.
4.1.3.1 Mg2+ increase of activity Ricinus communis
4.1.3.1 Mg2+ increase of activity Helianthus annuus
4.1.3.1 Mg2+ increase of activity Linum usitatissimum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 28500
-
 4 * 28500 Helianthus annuus
4.1.3.1 66000
-
 4 * 66000, SDS-PAGE Lupinus sp.
4.1.3.1 66000
-
 4 * 66000, SDS-PAGE Pinus pinea
4.1.3.1 114000
-
-
 Helianthus annuus
4.1.3.1 260000
-
-
 Lupinus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Lupinus sp. glyoxylate cycle ?
-
 ?
4.1.3.1 isocitrate Ricinus communis glyoxylate cycle ?
-
 ?
4.1.3.1 isocitrate Cucumis sativus glyoxylate cycle ?
-
 ?
4.1.3.1 isocitrate Helianthus annuus glyoxylate cycle ?
-
 ?
4.1.3.1 isocitrate Linum usitatissimum glyoxylate cycle ?
-
 ?
4.1.3.1 isocitrate Citrullus lanatus subsp. vulgaris glyoxylate cycle ?
-
 ?
4.1.3.1 isocitrate Pinus densiflora glyoxylate cycle ?
-
 ?
4.1.3.1 isocitrate Pinus pinea glyoxylate cycle ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Citrullus lanatus subsp. vulgaris
-
-
-
4.1.3.1 Cucumis sativus
-
 cucumber
-
4.1.3.1 Helianthus annuus
-
 sunflower
-
4.1.3.1 Linum usitatissimum
-
-
-
4.1.3.1 Lupinus sp.
-
-
-
4.1.3.1 Pinus densiflora
-
-
-
4.1.3.1 Pinus pinea
-
-
-
4.1.3.1 Ricinus communis
-
 castor bean
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 (2R,3S)-isocitrate
-
 Cucumis sativus succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Lupinus sp. succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Ricinus communis succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Cucumis sativus succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Helianthus annuus succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Linum usitatissimum succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Citrullus lanatus subsp. vulgaris succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Pinus densiflora succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Pinus pinea succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate glyoxylate cycle Lupinus sp. ?
-
 ?
4.1.3.1 isocitrate glyoxylate cycle Ricinus communis ?
-
 ?
4.1.3.1 isocitrate glyoxylate cycle Cucumis sativus ?
-
 ?
4.1.3.1 isocitrate glyoxylate cycle Helianthus annuus ?
-
 ?
4.1.3.1 isocitrate glyoxylate cycle Linum usitatissimum ?
-
 ?
4.1.3.1 isocitrate glyoxylate cycle Citrullus lanatus subsp. vulgaris ?
-
 ?
4.1.3.1 isocitrate glyoxylate cycle Pinus densiflora ?
-
 ?
4.1.3.1 isocitrate glyoxylate cycle Pinus pinea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.1 tetramer
-
 Ricinus communis
4.1.3.1 tetramer
-
 Cucumis sativus
4.1.3.1 tetramer
-
 Citrullus lanatus subsp. vulgaris
4.1.3.1 tetramer 4 * 28500 Helianthus annuus
4.1.3.1 tetramer 4 * 66000, SDS-PAGE Lupinus sp.
4.1.3.1 tetramer 4 * 66000, SDS-PAGE Pinus pinea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.1 6.8
-
-
 Lupinus sp.
4.1.3.1 7.3 7.5
-
 Pinus pinea
4.1.3.1 7.35 7.6
-
 Helianthus annuus