BRENDA - Enzyme Database

Isocitrate lyase from higher plants

Giachetti, E.G.; Pinzauti, G.; Bonaccorsi, R.; Vincenzini, M.T.; Vanni, P.; Phytochemistry 26, 2439-2446 (1987)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Citrullus lanatus subsp. vulgaris
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Cucumis sativus
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Helianthus annuus
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Linum usitatissimum
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Lupinus sp.
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Pinus densiflora
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Pinus pinea
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Ricinus communis
General Stability
EC Number
General Stability
Organism
4.1.3.1
most isocitrases very unstable, bacterial enzyme more stable than plant enzyme
Helianthus annuus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
citrate
-
Helianthus annuus
4.1.3.1
citrate
-
Ricinus communis
4.1.3.1
glycolate
-
Helianthus annuus
4.1.3.1
glycolate
-
Lupinus sp.
4.1.3.1
glycolate
-
Pinus pinea
4.1.3.1
glyoxylate
-
Lupinus sp.
4.1.3.1
glyoxylate
-
Pinus pinea
4.1.3.1
glyoxylate
-
Linum usitatissimum
4.1.3.1
malate
-
Lupinus sp.
4.1.3.1
malate
-
Ricinus communis
4.1.3.1
Maleate
-
Pinus densiflora
4.1.3.1
Maleate
-
Ricinus communis
4.1.3.1
malonate
-
Citrullus lanatus subsp. vulgaris
4.1.3.1
malonate
-
Lupinus sp.
4.1.3.1
malonate
-
Ricinus communis
4.1.3.1
malonate
-
Linum usitatissimum
4.1.3.1
methylenesuccinate
-
Citrullus lanatus subsp. vulgaris
4.1.3.1
methylenesuccinate
-
Linum usitatissimum
4.1.3.1
methylenesuccinate
-
Lupinus sp.
4.1.3.1
methylenesuccinate
-
Pinus densiflora
4.1.3.1
methylenesuccinate
-
Pinus pinea
4.1.3.1
oxalate
-
Citrullus lanatus subsp. vulgaris
4.1.3.1
oxalate
-
Helianthus annuus
4.1.3.1
oxalate
-
Lupinus sp.
4.1.3.1
oxalate
-
Pinus densiflora
4.1.3.1
oxalate
-
Pinus pinea
4.1.3.1
phosphoenolpyruvate
-
Linum usitatissimum
4.1.3.1
phosphoenolpyruvate
-
Lupinus sp.
4.1.3.1
phosphoenolpyruvate
-
Pinus densiflora
4.1.3.1
succinate
-
Helianthus annuus
4.1.3.1
succinate
-
Linum usitatissimum
4.1.3.1
succinate
-
Lupinus sp.
4.1.3.1
succinate
-
Pinus densiflora
4.1.3.1
succinate
-
Pinus pinea
4.1.3.1
succinate
-
Ricinus communis
4.1.3.1
Tartrate
-
Citrullus lanatus subsp. vulgaris
4.1.3.1
Tartrate
-
Lupinus sp.
4.1.3.1
Tartrate
-
Pinus densiflora
4.1.3.1
Tartrate
-
Linum usitatissimum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.032
-
isocitrate
phosphate, pH 6.0
Helianthus annuus
4.1.3.1
0.033
-
Ds-isocitrate
HEPES, pH 7.0
Pinus pinea
4.1.3.1
0.035
-
isocitrate
imidazole, pH 7.0
Lupinus sp.
4.1.3.1
0.039
-
isocitrate
MOPS, pH 6.8
Cucumis sativus
4.1.3.1
0.1
-
threo-Ds-isocitrate
Hepes, pH 7.4
Cucumis sativus
4.1.3.1
0.11
-
isocitrate
TRA, pH 7.0
Pinus pinea
4.1.3.1
0.25
-
isocitrate
MOPS, pH 7.5
Citrullus lanatus subsp. vulgaris
4.1.3.1
0.29
-
isocitrate
phosphate, pH 7.5
Linum usitatissimum
4.1.3.1
0.3
-
isocitrate
phosphate, pH 7.0
Ricinus communis
4.1.3.1
0.66
-
isocitrate
Tris, pH 7.6
Pinus densiflora
4.1.3.1
0.67
-
isocitrate
phosphate, pH 7.5
Helianthus annuus
4.1.3.1
1
-
isocitrate
phosphate, pH 6.8
Ricinus communis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.3.1
glyoxysome
matrix
Cucumis sativus
9514
-
4.1.3.1
glyoxysome
matrix
Helianthus annuus
9514
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
maximal activity at 5-6 mM
Cucumis sativus
4.1.3.1
Mg2+
increase of activity
Helianthus annuus
4.1.3.1
Mg2+
increase of activity; maximal activity at 3.7 mM
Linum usitatissimum
4.1.3.1
Mg2+
increase of activity; maximal activity at 5 mM
Lupinus sp.
4.1.3.1
Mg2+
-
Pinus densiflora
4.1.3.1
Mg2+
maximal activity at 6 mM
Pinus pinea
4.1.3.1
Mg2+
increase of activity
Ricinus communis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
28500
-
4 * 28500
Helianthus annuus
4.1.3.1
66000
-
4 * 66000, SDS-PAGE
Lupinus sp.
4.1.3.1
66000
-
4 * 66000, SDS-PAGE
Pinus pinea
4.1.3.1
114000
-
-
Helianthus annuus
4.1.3.1
260000
-
-
Lupinus sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Lupinus sp.
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Ricinus communis
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Cucumis sativus
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Helianthus annuus
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Linum usitatissimum
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Citrullus lanatus subsp. vulgaris
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Pinus densiflora
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Pinus pinea
glyoxylate cycle
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Citrullus lanatus subsp. vulgaris
-
-
-
4.1.3.1
Cucumis sativus
-
cucumber
-
4.1.3.1
Helianthus annuus
-
sunflower
-
4.1.3.1
Linum usitatissimum
-
-
-
4.1.3.1
Lupinus sp.
-
-
-
4.1.3.1
Pinus densiflora
-
-
-
4.1.3.1
Pinus pinea
-
-
-
4.1.3.1
Ricinus communis
-
castor bean
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33138
Cucumis sativus
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33138
Lupinus sp.
succinate + glyoxylate
-
33138
Lupinus sp.
-
4.1.3.1
isocitrate
-
33138
Ricinus communis
succinate + glyoxylate
-
33138
Ricinus communis
-
4.1.3.1
isocitrate
-
33138
Cucumis sativus
succinate + glyoxylate
-
33138
Cucumis sativus
-
4.1.3.1
isocitrate
-
33138
Helianthus annuus
succinate + glyoxylate
-
33138
Helianthus annuus
-
4.1.3.1
isocitrate
-
33138
Linum usitatissimum
succinate + glyoxylate
-
33138
Linum usitatissimum
-
4.1.3.1
isocitrate
-
33138
Citrullus lanatus subsp. vulgaris
succinate + glyoxylate
-
33138
Citrullus lanatus subsp. vulgaris
-
4.1.3.1
isocitrate
-
33138
Pinus densiflora
succinate + glyoxylate
-
33138
Pinus densiflora
-
4.1.3.1
isocitrate
-
33138
Pinus pinea
succinate + glyoxylate
-
33138
Pinus pinea
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Lupinus sp.
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Ricinus communis
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Cucumis sativus
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Helianthus annuus
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Linum usitatissimum
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Pinus densiflora
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Pinus pinea
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
-
Citrullus lanatus subsp. vulgaris
4.1.3.1
tetramer
-
Cucumis sativus
4.1.3.1
tetramer
4 * 28500
Helianthus annuus
4.1.3.1
tetramer
4 * 66000, SDS-PAGE
Lupinus sp.
4.1.3.1
tetramer
4 * 66000, SDS-PAGE
Pinus pinea
4.1.3.1
tetramer
-
Ricinus communis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.8
-
-
Lupinus sp.
4.1.3.1
7.3
7.5
-
Pinus pinea
4.1.3.1
7.35
7.6
-
Helianthus annuus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Citrullus lanatus subsp. vulgaris
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Cucumis sativus
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Helianthus annuus
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Linum usitatissimum
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Lupinus sp.
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Pinus densiflora
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Pinus pinea
4.1.3.1
additional information
no evidence that isocitrate lyase contains coenzyme
Ricinus communis
General Stability (protein specific)
EC Number
General Stability
Organism
4.1.3.1
most isocitrases very unstable, bacterial enzyme more stable than plant enzyme
Helianthus annuus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
citrate
-
Helianthus annuus
4.1.3.1
citrate
-
Ricinus communis
4.1.3.1
glycolate
-
Helianthus annuus
4.1.3.1
glycolate
-
Lupinus sp.
4.1.3.1
glycolate
-
Pinus pinea
4.1.3.1
glyoxylate
-
Lupinus sp.
4.1.3.1
glyoxylate
-
Pinus pinea
4.1.3.1
glyoxylate
-
Linum usitatissimum
4.1.3.1
malate
-
Lupinus sp.
4.1.3.1
malate
-
Ricinus communis
4.1.3.1
Maleate
-
Pinus densiflora
4.1.3.1
Maleate
-
Ricinus communis
4.1.3.1
malonate
-
Citrullus lanatus subsp. vulgaris
4.1.3.1
malonate
-
Lupinus sp.
4.1.3.1
malonate
-
Ricinus communis
4.1.3.1
malonate
-
Linum usitatissimum
4.1.3.1
methylenesuccinate
-
Citrullus lanatus subsp. vulgaris
4.1.3.1
methylenesuccinate
-
Linum usitatissimum
4.1.3.1
methylenesuccinate
-
Lupinus sp.
4.1.3.1
methylenesuccinate
-
Pinus densiflora
4.1.3.1
methylenesuccinate
-
Pinus pinea
4.1.3.1
oxalate
-
Citrullus lanatus subsp. vulgaris
4.1.3.1
oxalate
-
Helianthus annuus
4.1.3.1
oxalate
-
Lupinus sp.
4.1.3.1
oxalate
-
Pinus densiflora
4.1.3.1
oxalate
-
Pinus pinea
4.1.3.1
phosphoenolpyruvate
-
Linum usitatissimum
4.1.3.1
phosphoenolpyruvate
-
Lupinus sp.
4.1.3.1
phosphoenolpyruvate
-
Pinus densiflora
4.1.3.1
succinate
-
Helianthus annuus
4.1.3.1
succinate
-
Linum usitatissimum
4.1.3.1
succinate
-
Lupinus sp.
4.1.3.1
succinate
-
Pinus densiflora
4.1.3.1
succinate
-
Pinus pinea
4.1.3.1
succinate
-
Ricinus communis
4.1.3.1
Tartrate
-
Citrullus lanatus subsp. vulgaris
4.1.3.1
Tartrate
-
Lupinus sp.
4.1.3.1
Tartrate
-
Pinus densiflora
4.1.3.1
Tartrate
-
Linum usitatissimum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.032
-
isocitrate
phosphate, pH 6.0
Helianthus annuus
4.1.3.1
0.033
-
Ds-isocitrate
HEPES, pH 7.0
Pinus pinea
4.1.3.1
0.035
-
isocitrate
imidazole, pH 7.0
Lupinus sp.
4.1.3.1
0.039
-
isocitrate
MOPS, pH 6.8
Cucumis sativus
4.1.3.1
0.1
-
threo-Ds-isocitrate
Hepes, pH 7.4
Cucumis sativus
4.1.3.1
0.11
-
isocitrate
TRA, pH 7.0
Pinus pinea
4.1.3.1
0.25
-
isocitrate
MOPS, pH 7.5
Citrullus lanatus subsp. vulgaris
4.1.3.1
0.29
-
isocitrate
phosphate, pH 7.5
Linum usitatissimum
4.1.3.1
0.3
-
isocitrate
phosphate, pH 7.0
Ricinus communis
4.1.3.1
0.66
-
isocitrate
Tris, pH 7.6
Pinus densiflora
4.1.3.1
0.67
-
isocitrate
phosphate, pH 7.5
Helianthus annuus
4.1.3.1
1
-
isocitrate
phosphate, pH 6.8
Ricinus communis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.3.1
glyoxysome
matrix
Cucumis sativus
9514
-
4.1.3.1
glyoxysome
matrix
Helianthus annuus
9514
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
maximal activity at 5-6 mM
Cucumis sativus
4.1.3.1
Mg2+
increase of activity
Helianthus annuus
4.1.3.1
Mg2+
increase of activity; maximal activity at 3.7 mM
Linum usitatissimum
4.1.3.1
Mg2+
increase of activity; maximal activity at 5 mM
Lupinus sp.
4.1.3.1
Mg2+
-
Pinus densiflora
4.1.3.1
Mg2+
maximal activity at 6 mM
Pinus pinea
4.1.3.1
Mg2+
increase of activity
Ricinus communis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
28500
-
4 * 28500
Helianthus annuus
4.1.3.1
66000
-
4 * 66000, SDS-PAGE
Lupinus sp.
4.1.3.1
66000
-
4 * 66000, SDS-PAGE
Pinus pinea
4.1.3.1
114000
-
-
Helianthus annuus
4.1.3.1
260000
-
-
Lupinus sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Lupinus sp.
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Ricinus communis
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Cucumis sativus
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Helianthus annuus
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Linum usitatissimum
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Citrullus lanatus subsp. vulgaris
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Pinus densiflora
glyoxylate cycle
?
-
-
-
4.1.3.1
isocitrate
Pinus pinea
glyoxylate cycle
?
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
(2R,3S)-isocitrate
-
33138
Cucumis sativus
succinate + glyoxylate
-
-
-
-
4.1.3.1
isocitrate
-
33138
Lupinus sp.
succinate + glyoxylate
-
33138
Lupinus sp.
-
4.1.3.1
isocitrate
-
33138
Ricinus communis
succinate + glyoxylate
-
33138
Ricinus communis
-
4.1.3.1
isocitrate
-
33138
Cucumis sativus
succinate + glyoxylate
-
33138
Cucumis sativus
-
4.1.3.1
isocitrate
-
33138
Helianthus annuus
succinate + glyoxylate
-
33138
Helianthus annuus
-
4.1.3.1
isocitrate
-
33138
Linum usitatissimum
succinate + glyoxylate
-
33138
Linum usitatissimum
-
4.1.3.1
isocitrate
-
33138
Citrullus lanatus subsp. vulgaris
succinate + glyoxylate
-
33138
Citrullus lanatus subsp. vulgaris
-
4.1.3.1
isocitrate
-
33138
Pinus densiflora
succinate + glyoxylate
-
33138
Pinus densiflora
-
4.1.3.1
isocitrate
-
33138
Pinus pinea
succinate + glyoxylate
-
33138
Pinus pinea
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Lupinus sp.
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Ricinus communis
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Cucumis sativus
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Helianthus annuus
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Linum usitatissimum
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Citrullus lanatus subsp. vulgaris
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Pinus densiflora
?
-
-
-
-
4.1.3.1
isocitrate
glyoxylate cycle
33138
Pinus pinea
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
-
Citrullus lanatus subsp. vulgaris
4.1.3.1
tetramer
-
Cucumis sativus
4.1.3.1
tetramer
4 * 28500
Helianthus annuus
4.1.3.1
tetramer
4 * 66000, SDS-PAGE
Lupinus sp.
4.1.3.1
tetramer
4 * 66000, SDS-PAGE
Pinus pinea
4.1.3.1
tetramer
-
Ricinus communis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.8
-
-
Lupinus sp.
4.1.3.1
7.3
7.5
-
Pinus pinea
4.1.3.1
7.35
7.6
-
Helianthus annuus