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Literature summary extracted from

  • Tsukamoto, C.; Ejiri, S.I.; Katsumata, T.
    Purification and some properties of isocitrate lyase from the pollen of Pinus densiflora Sieb. et Zucc. (1986), Agric. Biol. Chem., 50, 409-416.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.1.3.1 extremely unstable in absence of Mg2+ or EDTA, glycerol or sucrose could not stabilize Pinus densiflora

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 2-mercaptoethanol
-
 Pinus densiflora
4.1.3.1 3-phosphoglycerate 29% inhibition at 1 mM Pinus densiflora
4.1.3.1 ADP 20% inhibition at 2 mM Pinus densiflora
4.1.3.1 ATP 35% inhibition at 2 mM Pinus densiflora
4.1.3.1 CoCl2
-
 Pinus densiflora
4.1.3.1 CuCl2
-
 Pinus densiflora
4.1.3.1 cysteine
-
 Pinus densiflora
4.1.3.1 dithiothreitol
-
 Pinus densiflora
4.1.3.1 FeCl2
-
 Pinus densiflora
4.1.3.1 glutathione
-
 Pinus densiflora
4.1.3.1 Maleate 66% inhibition at 0.5 mM Pinus densiflora
4.1.3.1 methylenesuccinate 87% inhibition at 0.03 mM Pinus densiflora
4.1.3.1 oxalate 71% inhibition at 0.5 mM Pinus densiflora
4.1.3.1 p-chloromercuribenzoate reversible by cysteine Pinus densiflora
4.1.3.1 PbCl2
-
 Pinus densiflora
4.1.3.1 phosphoenolpyruvate 53% inhibition at 1 mM Pinus densiflora
4.1.3.1 succinate 7% inhibition at 0.5 mM Pinus densiflora
4.1.3.1 sulfhydryl compounds
-
 Pinus densiflora
4.1.3.1 Tartrate 33% inhibition at 0.5 mM Pinus densiflora

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Ba2+ Ba2+ less effective activator than Mg2+ Pinus densiflora
4.1.3.1 Mg2+ essential for activity, optimal concentration 3 mM Pinus densiflora

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 65000
-
 3 * 65000, SDS-PAGE Pinus densiflora
4.1.3.1 200000
-
 gel filtration, glycerol density gradient Pinus densiflora

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Pinus densiflora
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1
-
 Pinus densiflora

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.1 pollen
-
 Pinus densiflora
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Pinus densiflora succinate + glyoxylate
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.3.1 trimer 3 * 65000, SDS-PAGE Pinus densiflora

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.1 40
-
-
 Pinus densiflora

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.1 additional information
-
 more heat labile at low concentrations Pinus densiflora
4.1.3.1 50
-
 completely inactivated after 5 min Pinus densiflora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.1 7.6
-
-
 Pinus densiflora

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.3.1 7.2 8.2
-
 Pinus densiflora

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.3.1 7 8.5
-
 Pinus densiflora