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Literature summary extracted from
- Purification and properties of beta-glucosidase from Candida pelliculosa var. acetaetherius (1985), Agric. Biol. Chem., 49, 779-784.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | beta-D-glucose | competitive inhibition, Ki: 6.5 mM | Wickerhamomyces anomalus |  |
| 3.2.1.21 | Co2+ | 40% inhibition at 1 mM | Wickerhamomyces anomalus | |
| 3.2.1.21 | Cu2+ | nearly complete inhibition at 1 mM | Wickerhamomyces anomalus | |
| 3.2.1.21 | Hg2+ | complete inhibition at 1 mM | Wickerhamomyces anomalus | |
| 3.2.1.21 | p-chloromercuribenzoic acid | strong inhibition at 1 mM | Wickerhamomyces anomalus | |
| 3.2.1.21 | SDS | 79% inhibition at 1 mM | Wickerhamomyces anomalus | |
| 3.2.1.21 | Zn2+ | 67% inhibition at 1 mM | Wickerhamomyces anomalus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.5 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Wickerhamomyces anomalus | |
| 3.2.1.21 | 19.2 | - |
Salicin | - |
Wickerhamomyces anomalus | |
| 3.2.1.21 | 37 | - |
cellobiose | - |
Wickerhamomyces anomalus | |
| 3.2.1.21 | 44.4 | - |
methyl-beta-D-glucoside | - |
Wickerhamomyces anomalus | |
| 3.2.1.21 | 100 | - |
gentiobiose | - |
Wickerhamomyces anomalus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | intracellular | - |
Wickerhamomyces anomalus | 5622 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 90000 | - |
4 * 90000, SDS-PAGE | Wickerhamomyces anomalus |
| 3.2.1.21 | 360000 | - |
gel filtration | Wickerhamomyces anomalus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Wickerhamomyces anomalus | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Wickerhamomyces anomalus | low activity | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Wickerhamomyces anomalus | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Wickerhamomyces anomalus | - |
var. acetaetherius | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | glycoprotein | - |
Wickerhamomyces anomalus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | to homogeneity, chromatography steps | Wickerhamomyces anomalus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | cell culture | - |
Wickerhamomyces anomalus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 207 | - |
- |
Wickerhamomyces anomalus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | low activity | Wickerhamomyces anomalus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Wickerhamomyces anomalus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 2-nitrophenyl beta-D-galactopyranoside + H2O | low activity | Wickerhamomyces anomalus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | specific for beta-configuration of sugars | Wickerhamomyces anomalus | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Wickerhamomyces anomalus | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Wickerhamomyces anomalus | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity | Wickerhamomyces anomalus | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Wickerhamomyces anomalus | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Wickerhamomyces anomalus | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | gentiobiose + H2O | - |
Wickerhamomyces anomalus | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | methyl-beta-D-glucoside + H2O | very low activity | Wickerhamomyces anomalus | beta-D-glucose + methanol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | tetramer | 4 * 90000, SDS-PAGE | Wickerhamomyces anomalus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
- |
Wickerhamomyces anomalus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 6.5 | - |
- |
Wickerhamomyces anomalus |
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Release 2023.1 (January 2023)
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