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Literature summary extracted from
- Purification and characterization of the extracellular beta-glucosidase produced by Candida wickerhamii (1985), Arch. Biochem. Biophys., 243, 515-522.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 4.17 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Nakazawaea wickerhamii |  |
| 3.2.1.21 | 67.4 | - |
cellobiose | - |
Nakazawaea wickerhamii | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | extracellular | - |
Nakazawaea wickerhamii | - |
- |
| 3.2.1.21 | intracellular | - |
Nakazawaea wickerhamii | 5622 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 94000 | - |
2 * 94000, SDS-PAGE | Nakazawaea wickerhamii |
| 3.2.1.21 | 198000 | - |
gel filtration | Nakazawaea wickerhamii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Nakazawaea wickerhamii | cellobiose | beta-D-glucose + D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Nakazawaea wickerhamii | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | glycoprotein | 30.5%, w/w, carbohydrate content | Nakazawaea wickerhamii |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | to homogeneity, chromatography steps | Nakazawaea wickerhamii |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | cell culture | - |
Nakazawaea wickerhamii | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 180 | - |
- |
Nakazawaea wickerhamii |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.21 | 4°C or -20°C, without loss in activity for more than 6 months | Nakazawaea wickerhamii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Nakazawaea wickerhamii | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Nakazawaea wickerhamii | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Nakazawaea wickerhamii | 2 beta-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | dimer | 2 * 94000, SDS-PAGE | Nakazawaea wickerhamii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 35 | - |
inactivation above | Nakazawaea wickerhamii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4 | 5 | - |
Nakazawaea wickerhamii |
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Release 2023.1 (January 2023)
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