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Literature summary extracted from
- Cellulases of a mutant strain of Trichoderma viride QM 9414 (1988), Methods Enzymol., 160, 243-251.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.4 | additional information | - |
additional information | - |
Trichoderma viride |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 45000 | - |
x * 45000, endoglucanase II, SDS-PAGE | Trichoderma viride |
| 3.2.1.4 | 50000 | - |
x * 50000, endoglucanase IV, SDS-PAGE | Trichoderma viride |
| 3.2.1.4 | 50000 | - |
x * 50000, endoglucanase I, SDS-PAGE | Trichoderma viride |
| 3.2.1.4 | 52000 | - |
x * 52000, endoglucanase VI, SDS-PAGE | Trichoderma viride |
| 3.2.1.4 | 57000 | - |
x * 57000, endoglucanase V, SDS-PAGE | Trichoderma viride |
| 3.2.1.4 | 585000 | - |
x * 585000, endoglucanase III, SDS-PAGE | Trichoderma viride |
| 3.2.1.21 | 76000 | - |
gel filtration | Trichoderma viride |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | xylan + H2O | Trichoderma viride | moderately active | beta-D-glucose + ? | - |
? |  |
| 3.2.1.21 | xylan + H2O | Trichoderma viride QM 9414 | moderately active | beta-D-glucose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.4 | Trichoderma viride | - |
QM 9414, mutant strain | - |
| 3.2.1.4 | Trichoderma viride QM 9414 | - |
QM 9414, mutant strain | - |
| 3.2.1.21 | Trichoderma viride | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | glycoprotein | endoglucanases I, II, III, V, and VI are glycoproteins | Trichoderma viride |
| 3.2.1.4 | no modification | endoglucanase IV contains no carbohydrate | Trichoderma viride |
| 3.2.1.21 | glycoprotein | - |
Trichoderma viride |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | endoglucanase I, II, III, IV, V and VI | Trichoderma viride |
| 3.2.1.21 | to homogeneity, crude enzyme preparation by further chromatography steps | Trichoderma viride |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | commercial preparation | - |
Trichoderma viride | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 0.4 | - |
- |
Trichoderma viride |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.4 | avicel + H2O | - |
Trichoderma viride | ? | - |
? | |
| 3.2.1.4 | carboxymethylcellulose + H2O | - |
Trichoderma viride | additional information | - |
? | |
| 3.2.1.4 | cellulose + H2O | acid-swollen | Trichoderma viride | ? | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Trichoderma viride | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Trichoderma viride QM 9414 | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | highly active | Trichoderma viride | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | highly active | Trichoderma viride QM 9414 | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | Avicel + H2O | moderately active | Trichoderma viride | beta-D-glucose + ? | - |
? | |
| 3.2.1.21 | Avicel + H2O | moderately active | Trichoderma viride QM 9414 | beta-D-glucose + ? | - |
? | |
| 3.2.1.21 | carboxymethyl-cellulose + H2O | low activity | Trichoderma viride | beta-D-glucose + ? | - |
? | |
| 3.2.1.21 | carboxymethyl-cellulose + H2O | low activity | Trichoderma viride QM 9414 | beta-D-glucose + ? | - |
? | |
| 3.2.1.21 | xylan + H2O | moderately active | Trichoderma viride | beta-D-glucose + ? | - |
? | |
| 3.2.1.21 | xylan + H2O | moderately active | Trichoderma viride QM 9414 | beta-D-glucose + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | ? | x * 50000, endoglucanase IV, SDS-PAGE | Trichoderma viride |
| 3.2.1.4 | ? | x * 45000, endoglucanase II, SDS-PAGE | Trichoderma viride |
| 3.2.1.4 | ? | x * 52000, endoglucanase VI, SDS-PAGE | Trichoderma viride |
| 3.2.1.4 | ? | x * 57000, endoglucanase V, SDS-PAGE | Trichoderma viride |
| 3.2.1.4 | ? | x * 585000, endoglucanase III, SDS-PAGE | Trichoderma viride |
| 3.2.1.4 | ? | x * 50000, endoglucanase I, SDS-PAGE | Trichoderma viride |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | beta-glucosidase I | - |
Trichoderma viride |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 4 | - |
endoglucanase II | Trichoderma viride |
| 3.2.1.4 | 4.5 | - |
endoglucanase V and VI | Trichoderma viride |
| 3.2.1.4 | 5 | - |
endoglucanase I and III | Trichoderma viride |
| 3.2.1.4 | 5.5 | - |
endoglucanase IV | Trichoderma viride |
| 3.2.1.21 | 4.7 | - |
- |
Trichoderma viride |
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