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Literature summary extracted from
- Immobilization of beta-glucosidase from Myceliophthora thermophila D-14 (1989), Enzyme Microb. Technol., 11, 431-435.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 4.5 | - |
4-nitrophenyl-beta-D-glucopyranoside | non-immobilized soluble enzyme | Thermothelomyces heterothallicus |  |
| 3.2.1.21 | 5 | - |
4-nitrophenyl-beta-D-glucopyranoside | immobilized enzyme to polyacrylamide gel | Thermothelomyces heterothallicus | |
| 3.2.1.21 | 5.2 | - |
4-nitrophenyl-beta-D-glucopyranoside | immobilized enzyme to CNBr-Sepharose | Thermothelomyces heterothallicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | extracellular | - |
Thermothelomyces heterothallicus | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Thermothelomyces heterothallicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | partial | Thermothelomyces heterothallicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | cell culture | - |
Thermothelomyces heterothallicus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermothelomyces heterothallicus | 4-nitrophenol + D-glucopyranose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 60 | - |
non-immobilized enzyme preparation | Thermothelomyces heterothallicus |
| 3.2.1.21 | 70 | - |
immobilized enzyme to CNBr-Sepharose | Thermothelomyces heterothallicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 60 | - |
stable for 1 h, immobilized enzyme shows better stability | Thermothelomyces heterothallicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4.8 | - |
for non-immobilized and immobilized enzyme preparations | Thermothelomyces heterothallicus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4.5 | 6 | stable within, soluble and immobilized enzyme preparations | Thermothelomyces heterothallicus |
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