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Literature summary extracted from

  • Roessner, C.A.; Spencer, J.B.; Ozaki, S.; Min, C.; Atshaves, B.P.; Nayar, P.; Anousis, N.; Stolowich, N.J.; Holderman, M.T.; Scott, A.I.
    Overexpression in Escherichia coli of 12 vitamin B12 biosynthetic enzymes (1995), Protein Expr. Purif., 6, 155-163.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.54 functional overexpression of gene cobK together with the other 11 of the first 12 enzymes involved in biosynthesis of cobalamin, vitamin B12, from aminolevulinic acid, in Escherichia coli strains TB1 and BL21(DE3) Pseudomonas denitrificans (nom. rej.)
1.14.13.83 overexpressed in recombinant strains of Escherichia coli Pseudomonas denitrificans (nom. rej.)
2.1.1.130 expression in Escherichia coli Pseudomonas denitrificans (nom. rej.)
2.1.1.132 overexpression in Escherichia coli Pseudomonas denitrificans (nom. rej.)
2.1.1.133 overexpression in Escherichia coli Pseudomonas denitrificans (nom. rej.)
2.1.1.152 expression in Escherichia coli Pseudomonas denitrificans (nom. rej.)
5.4.99.61 overexpression in Escherichia coli Pseudomonas denitrificans (nom. rej.)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.83 Fe2+ contains Fe4S4 Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.54 26000
-
 x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
1.3.1.54 28095
-
 x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
1.14.13.83 46690
-
 calculated from the gene sequence Pseudomonas denitrificans (nom. rej.)
1.14.13.83 48000
-
 SDS-PAGE Pseudomonas denitrificans (nom. rej.)
2.1.1.130 25895
-
 2 * 25895, calculation from nucleotide sequence Pseudomonas denitrificans (nom. rej.)
2.1.1.132 42952
-
 2 * 42952, calculation from nucleotide sequence Pseudomonas denitrificans (nom. rej.)
2.1.1.133 26862
-
 2 * 26862, calculation from nucleotide sequence Pseudomonas denitrificans (nom. rej.)
2.1.1.152 28944
-
 2 * 28944, calculation from nucleotide sequence Pseudomonas denitrificans (nom. rej.)
2.1.1.152 30000
-
 2 * 30000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
5.4.99.61 22013
-
 2 * 22013, calculation from nucleotide sequence Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.54 precorrin-6A + NADPH Pseudomonas denitrificans (nom. rej.) enzyme catalyzes step 10 in the biosynthesis of cobalamine, vitamin B12, pathway overview precorrin-6B + NADP+
-
 ?
1.14.13.83 additional information Pseudomonas denitrificans (nom. rej.) biosynthesis of vitamin B12 ?
-
 ?
2.1.1.130 S-adenosyl-L-methionine + precorrin-2 Pseudomonas denitrificans (nom. rej.) enzyme is involved in the biosynthesis of vitamin B12 ?
-
 ?
2.1.1.131 S-Adenosyl-L-methionine + precorrin-3B Pseudomonas denitrificans (nom. rej.) the enzyme is involved in the cobalamin biosynthetic pathway ?
-
 ?
2.1.1.132 Adenosyl-L-methionine + precorrin-6Y Pseudomonas denitrificans (nom. rej.) enzyme of the vitamin B12 biosynthetic pathway ?
-
 ?
2.1.1.133 S-Adenosyl-L-methionine + precorrin-4 Pseudomonas denitrificans (nom. rej.) enzyme in the biosynthesis of vitamin B12 ?
-
 ?
2.1.1.152 S-adenosyl-L-methionine + precorrin-5 + H2O Pseudomonas denitrificans (nom. rej.) enzyme is involved in synthesis of hydrogenobyrinic acid S-adenosyl-L-homocysteine + precorrin-6A + acetate
-
 ?
5.4.99.61 Precorrin-8X Pseudomonas denitrificans (nom. rej.) the enzyme is involved in the cobalamin biosynthetic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.54 Pseudomonas denitrificans (nom. rej.)
-
-
-
1.14.13.83 Pseudomonas denitrificans (nom. rej.)
-
-
-
2.1.1.130 Pseudomonas denitrificans (nom. rej.)
-
-
-
2.1.1.131 Pseudomonas denitrificans (nom. rej.)
-
-
-
2.1.1.132 Pseudomonas denitrificans (nom. rej.)
-
-
-
2.1.1.133 Pseudomonas denitrificans (nom. rej.)
-
-
-
2.1.1.152 Pseudomonas denitrificans (nom. rej.)
-
-
-
5.4.99.61 Pseudomonas denitrificans (nom. rej.)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.54 recombinant soluble enzyme from Escherichia coli Pseudomonas denitrificans (nom. rej.)
1.14.13.83
-
 Pseudomonas denitrificans (nom. rej.)
2.1.1.130
-
 Pseudomonas denitrificans (nom. rej.)
2.1.1.133
-
 Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.54 precorrin-6A + NADPH
-
 Pseudomonas denitrificans (nom. rej.) precorrin-6B + NADP+
-
 ?
1.3.1.54 precorrin-6A + NADPH enzyme catalyzes step 10 in the biosynthesis of cobalamine, vitamin B12, pathway overview Pseudomonas denitrificans (nom. rej.) precorrin-6B + NADP+
-
 ?
1.14.13.83 additional information biosynthesis of vitamin B12 Pseudomonas denitrificans (nom. rej.) ?
-
 ?
1.14.13.83 precorrin-3 + NADH + H+ + O2
-
 Pseudomonas denitrificans (nom. rej.) precorrin-3x + NAD+ + H2O
-
 ir
2.1.1.130 S-adenosyl-L-methionine + precorrin-2
-
 Pseudomonas denitrificans (nom. rej.) S-adenosyl-L-homocysteine + precorrin-3A
-
 ?
2.1.1.130 S-adenosyl-L-methionine + precorrin-2 enzyme is involved in the biosynthesis of vitamin B12 Pseudomonas denitrificans (nom. rej.) ?
-
 ?
2.1.1.131 S-Adenosyl-L-methionine + precorrin-3B the enzyme is involved in the cobalamin biosynthetic pathway Pseudomonas denitrificans (nom. rej.) ?
-
 ?
2.1.1.132 Adenosyl-L-methionine + precorrin-6Y enzyme of the vitamin B12 biosynthetic pathway Pseudomonas denitrificans (nom. rej.) ?
-
 ?
2.1.1.133 S-adenosyl-L-methionine + precorrin-4
-
 Pseudomonas denitrificans (nom. rej.) S-adenosyl-L-homocysteine + precorrin-5
-
 ?
2.1.1.133 S-Adenosyl-L-methionine + precorrin-4 enzyme in the biosynthesis of vitamin B12 Pseudomonas denitrificans (nom. rej.) ?
-
 ?
2.1.1.152 S-adenosyl-L-methionine + precorrin-5 + H2O
-
 Pseudomonas denitrificans (nom. rej.) S-adenosyl-L-homocysteine + precorrin-6A + acetate
-
 ?
2.1.1.152 S-adenosyl-L-methionine + precorrin-5 + H2O enzyme is involved in synthesis of hydrogenobyrinic acid Pseudomonas denitrificans (nom. rej.) S-adenosyl-L-homocysteine + precorrin-6A + acetate
-
 ?
5.4.99.61 Precorrin-8X
-
 Pseudomonas denitrificans (nom. rej.) Hydrogenobyrinate
-
 ?
5.4.99.61 Precorrin-8X the enzyme is involved in the cobalamin biosynthetic pathway Pseudomonas denitrificans (nom. rej.) ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.54 ? x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
2.1.1.130 dimer 2 * 25895, calculation from nucleotide sequence Pseudomonas denitrificans (nom. rej.)
2.1.1.132 dimer 2 * 42952, calculation from nucleotide sequence Pseudomonas denitrificans (nom. rej.)
2.1.1.133 dimer 2 * 26862, calculation from nucleotide sequence Pseudomonas denitrificans (nom. rej.)
2.1.1.152 dimer 2 * 30000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
2.1.1.152 dimer 2 * 28944, calculation from nucleotide sequence Pseudomonas denitrificans (nom. rej.)
5.4.99.61 dimer 2 * 22013, calculation from nucleotide sequence Pseudomonas denitrificans (nom. rej.)

Synonyms

EC Number Synonyms Comment Organism
1.3.1.54 precorrin-6 reductase
-
 Pseudomonas denitrificans (nom. rej.)
1.14.13.83 CobG
-
 Pseudomonas denitrificans (nom. rej.)
1.14.13.83 precorrin-3X synthase
-
 Pseudomonas denitrificans (nom. rej.)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.54 30
-
 assay at Pseudomonas denitrificans (nom. rej.)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.54 8
-
 assay at Pseudomonas denitrificans (nom. rej.)

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.54 NADPH
-
 Pseudomonas denitrificans (nom. rej.)
1.14.13.83 NADH
-
 Pseudomonas denitrificans (nom. rej.)