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Literature summary extracted from

  • Wakayama, M.; Watanabe, E.; Takenaka, Y.; Miyamoto, Y.; Tau, Y.; Sakai, K.; Moriguchi, M.
    Cloning, expression, and nucleotide sequence of the N-acyl-D-aspartate amidohydrolase gene from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 (1995), J. Ferment. Bioeng., 80, 311-317.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.83 expression in Escherichia coli Achromobacter xylosoxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.83 53581
-
 1 * 53581, calculation from nucleotide sequence Achromobacter xylosoxidans

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.83 Achromobacter xylosoxidans
-
 subsp. xylosoxydans strain A-6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.83
-
 Achromobacter xylosoxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.83 2506
-
-
 Achromobacter xylosoxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.83 N-Acetyl-D-Asp + H2O
-
 Achromobacter xylosoxidans acetate + D-Asp
-
 ?
3.5.1.83 N-Formyl-D-Asp + H2O
-
 Achromobacter xylosoxidans Formate + D-Asp
-
 ?
3.5.1.83 N-Propanoyl-D-Asp + H2O
-
 Achromobacter xylosoxidans Propanoate + D-Asp
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.83 monomer 1 * 53581, calculation from nucleotide sequence Achromobacter xylosoxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.83 50
-
-
 Achromobacter xylosoxidans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.83 45
-
 10 min, stable up to Achromobacter xylosoxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.83 7.5 8
-
 Achromobacter xylosoxidans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.83 4 11 stable Achromobacter xylosoxidans