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Literature summary extracted from

  • Moriguchi, M.; Sakai, K.; Miyamoto, Y.; Wakayama, M.
    Production, purification, and characterization of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 (1993), Biosci. Biotechnol. Biochem., 57, 1149-1152.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.81 Co2+
-
 Achromobacter xylosoxidans
3.5.1.81 Cu2+
-
 Achromobacter xylosoxidans
3.5.1.81 D-Phe
-
 Achromobacter xylosoxidans
3.5.1.81 Fe2+
-
 Achromobacter xylosoxidans
3.5.1.81 N-Acetyl-D-allosoleucine
-
 Achromobacter xylosoxidans
3.5.1.81 Ni2+
-
 Achromobacter xylosoxidans
3.5.1.81 Zn2+
-
 Achromobacter xylosoxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.81 9.8
-
 N-Acetyl-D-Leu
-
 Achromobacter xylosoxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.81 52000
-
 1 * 52000, SDS-PAGE Achromobacter xylosoxidans
3.5.1.81 58000
-
 gel filtration Achromobacter xylosoxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.81 additional information Achromobacter xylosoxidans the best inducers are a poor substrate, N-acetyl-gamma-methyl-D-Leu, and an inhibitor, N-acetyl-D-alloisoleucine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.81 Achromobacter xylosoxidans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.81
-
 Achromobacter xylosoxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.81 990
-
-
 Achromobacter xylosoxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.81 additional information the best inducers are a poor substrate, N-acetyl-gamma-methyl-D-Leu, and an inhibitor, N-acetyl-D-alloisoleucine Achromobacter xylosoxidans ?
-
 ?
3.5.1.81 N-acetyl-D-Leu + H2O
-
 Achromobacter xylosoxidans acetate + D-Leu
-
 ?
3.5.1.81 N-Acetyl-D-Met + H2O
-
 Achromobacter xylosoxidans acetate + D-Met
-
 ?
3.5.1.81 N-Acetyl-D-norleucine + H2O
-
 Achromobacter xylosoxidans acetate + D-norleucine
-
 ?
3.5.1.81 N-Acetyl-D-Phe + H2O
-
 Achromobacter xylosoxidans acetate + D-Phe
-
 ?
3.5.1.81 N-Butyl-D-Leu + H2O
-
 Achromobacter xylosoxidans Butanoate + D-Leu
-
 ?
3.5.1.81 N-Formyl-D-Leu + H2O
-
 Achromobacter xylosoxidans Formate + D-Leu
-
 ?
3.5.1.81 N-Propionyl-D-Leu + H2O
-
 Achromobacter xylosoxidans Propanoate + D-Leu
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.81 monomer 1 * 52000, SDS-PAGE Achromobacter xylosoxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.81 50
-
-
 Achromobacter xylosoxidans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.81 40
-
 stable up to Achromobacter xylosoxidans
3.5.1.81 50
-
 pH 8.1, 10 min, optimal stability at Achromobacter xylosoxidans
3.5.1.81 50
-
 pH 7.4, 10 min, 5% loss of activity Achromobacter xylosoxidans
3.5.1.81 50
-
 pH 8.3, 10 min, 17% loss of activity Achromobacter xylosoxidans
3.5.1.81 60
-
 complete loss of activity above Achromobacter xylosoxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.81 7
-
-
 Achromobacter xylosoxidans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.81 7.4
-
 50°C, 10 min, 5% loss of activity Achromobacter xylosoxidans
3.5.1.81 8.1
-
 50°C, 10 min, optimal stability at Achromobacter xylosoxidans
3.5.1.81 8.3
-
 50°C, 10 min, 17% loss of activity Achromobacter xylosoxidans