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Literature summary extracted from

  • Johnson, G.D.; Hersh, L.B.
    Expression of meprin subunit precursors. Membrane anchoring through the beta subunit and mechanism of zymogen activation (1994), J. Biol. Chem., 269, 7682-7688.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.63 Trypsin peptidase activity of expressed meprin B precursor is absolutely dependent on activation by trypsin Rattus norvegicus
3.4.24.63 Trypsin activation is accompanied by reduction in size of 8 kDa Rattus norvegicus
3.4.24.63 Trypsin by limited proteolysis Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.63 rat, expressed in human kidney cell line 293, the expressed protein displays no detectable peptidase activity unless it is activated by removal of amino-terminal prosequence by limited trypsin digestion Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.63 membrane microvillar membrane, integral membrane protein Rattus norvegicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.63 74000
-
 x * 74000, deglycosylated enzyme, SDS-PAGE under reducing conditions Rattus norvegicus
3.4.24.63 84000
-
 x * 84000, expressed in human kidney 293 cells, deglycosylated form, SDS-PAGE under reducing conditions Rattus norvegicus
3.4.24.63 97000
-
 x * 97000, expressed in human kidney 293 cells, SDS-PAGE under reducing conditions Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.63 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.63 glycoprotein N-glycosidase F-sensitive Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.63 expressed in human kidney cell line 293, papain-solubilized Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.63 azocasein + H2O poor substrate Rattus norvegicus ?
-
 ?
3.4.24.63 additional information no substrates are glutaryl-Ala-Ala-Phe 4-methoxynaphthalamine 2-amide Rattus norvegicus ?
-
 ?
3.4.24.63 Succinyl-Ala-Ala-Ala 4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.24.63 Succinyl-Ala-Ala-Val-Ala 4-nitroanilide + H2O
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.63 oligomer x * 74000, deglycosylated enzyme, SDS-PAGE under reducing conditions Rattus norvegicus
3.4.24.63 oligomer x * 84000, expressed in human kidney 293 cells, deglycosylated form, SDS-PAGE under reducing conditions Rattus norvegicus
3.4.24.63 oligomer x * 97000, expressed in human kidney 293 cells, SDS-PAGE under reducing conditions Rattus norvegicus