Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Carvalho, K.M.; Joudiou, C.; Boussetta, H.; Leseney, A.M.; Cohen, P.
    A peptide-hormone-inactivating endopeptidase in Xenopus laevis skin secretion (1992), Proc. Natl. Acad. Sci. USA, 89, 84-88.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.4.24.60 Resistant to 1.5 M NaCl and 30% v/v methanol Xenopus laevis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.60 1,10-phenanthroline metalloendopeptidase character Xenopus laevis
3.4.24.60 Acetorphan less efficient than phosphoramidon Xenopus laevis
3.4.24.60 benzamidine not Xenopus laevis
3.4.24.60 EDTA metalloendopeptidase character Xenopus laevis
3.4.24.60 EGTA metalloendopeptidase character Xenopus laevis
3.4.24.60 Kelatorphan less efficient than phosphoramidon Xenopus laevis
3.4.24.60 additional information pepstatin, guanidylethylmercaptosuccinic acid (i.e. GEMSA), captopril, aprotinin, soybean trypsin inhibitor (i.e. STI), PMSF, IAA; retrothiorphan Xenopus laevis
3.4.24.60 N-ethylmaleimide not Xenopus laevis
3.4.24.60 p-chloromercuriphenylsulfonic acid not Xenopus laevis
3.4.24.60 Pentapeptides reproducing or mimicking the COOH-terminal sequence of ANF 24-28 ANF as substrate Xenopus laevis
3.4.24.60 phosphoramidon only at high concentrations, above 0.001 mM Xenopus laevis
3.4.24.60 thiorphan i.e. 3-mercapto-2-benzyl-propanoylglycine, only at high concentrations, above 0.001 mM Xenopus laevis
3.4.24.60 tosyl-Arg methyl ester not Xenopus laevis
3.4.24.60 [Leu5]enkephalin i.e. Tyr-Gly-Gly-Phe-Leu, ANF as substrate Xenopus laevis
3.4.24.60 [Leu5]enkephalinamide ANF as substrate Xenopus laevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.60 0.018
-
Somatostatin-14
-
Xenopus laevis
3.4.24.60 0.02
-
Substance P
-
Xenopus laevis
3.4.24.60 0.021
-
Neuromedin B
-
Xenopus laevis
3.4.24.60 0.022
-
Neuromedin C
-
Xenopus laevis
3.4.24.60 0.024
-
Litorin
-
Xenopus laevis
3.4.24.60 0.041
-
bradykinin
-
Xenopus laevis
3.4.24.60 0.05
-
(5-28)ANF
-
Xenopus laevis
3.4.24.60 0.063
-
angiotensin II
-
Xenopus laevis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.60 additional information metalloendopeptidase Xenopus laevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.60 100000
-
1 * 100000, Xenopus laevis, SDS-PAGE Xenopus laevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.60 additional information Xenopus laevis might be involved in inactivation of a great number of peptide hormones ?
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.24.60 Methanol resistant to 30% v/v methanol Xenopus laevis

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.60 Xenopus laevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.60
-
Xenopus laevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.60 sweat
-
Xenopus laevis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.60 0.1826
-
[D-Arg8]-kermit as substrate Xenopus laevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.60 (5-28)ANF + H2O i.e. atrial natriuretic factor, cleavage site: exclusively Ser25-Phe26 Xenopus laevis ANF(5-25) + Phe26-Arg27-Tyr28
-
?
3.4.24.60 angiotensin II + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe, cleavage site: Tyr4-Ile5 Xenopus laevis ?
-
?
3.4.24.60 bradykinin + H2O i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg, major cleavage site: Gly4-Phe5, minor site: Pro7-Pro8 Xenopus laevis ?
-
?
3.4.24.60 Litorin + H2O minor sites: Gly7-His8 Xenopus laevis ?
-
?
3.4.24.60 Litorin + H2O major cleavage sites: His8-Phe9 Xenopus laevis ?
-
?
3.4.24.60 additional information substrate specificity Xenopus laevis ?
-
?
3.4.24.60 additional information exhibits preference for cleavage on amino-terminal side of Phe, Leu or Ile in Xaa-Leu, Xaa-Phe or Xaa-Ile dipeptide Xenopus laevis ?
-
?
3.4.24.60 additional information no cleavage of analogous bonds in [Leu5]-enkephalin, oxytocin, vasopressin Xenopus laevis ?
-
?
3.4.24.60 additional information cleavage sites: Xaa-Phe, Xaa-Leu, Xaa-Ile Xenopus laevis ?
-
?
3.4.24.60 additional information [Leu5]enkephalin amide, minigastrin I Xenopus laevis ?
-
?
3.4.24.60 additional information might be involved in inactivation of a great number of peptide hormones Xenopus laevis ?
-
?
3.4.24.60 Neuromedin B + H2O i.e. Gly-Asn-Leu-Trp-Ala-Thr-Gly-His-Phe-Met-NH2, major cleavage site: His8-Phe9, minor site: Gly7-His8 Xenopus laevis ?
-
?
3.4.24.60 Neuromedin C + H2O Gly-Asn-His-Trp-Ala-Val-Gly-His-Leu-Met-NH2, major cleavage site: His8-Leu9, minor site: Gly7-His8 Xenopus laevis ?
-
?
3.4.24.60 Somatostatin-14 + H2O i.e. Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys, cleavage site: Phe6-Phe7 within disulfide bridged segment yielding one single product Xenopus laevis ?
-
?
3.4.24.60 Substance P + H2O major cleavage site: Gly9-Leu10, minor site: Phe7-Phe8 Xenopus laevis ?
-
?
3.4.24.60 [D-Arg8]-kermit + H2O model substrate, derivative of kermit i.e. Asp-Val-Asp-Glu-Arg-Asp-Val-Arg-Gly-Phe-Ala-Ser-Phe-Leu-NH2, cleavage site: Ser12-Phe13 Xenopus laevis ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.24.60 monomer 1 * 100000, Xenopus laevis, SDS-PAGE Xenopus laevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.60 7.5
-
-
Xenopus laevis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.60 6 8 about half-maximal activity at pH 6 and about 75% of maximal activity at pH 8 Xenopus laevis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.60 7.4
-
24 h stable Xenopus laevis