EC Number | General Stability | Organism |
---|---|---|
3.4.24.60 | Resistant to 1.5 M NaCl and 30% v/v methanol | Xenopus laevis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.60 | 1,10-phenanthroline | metalloendopeptidase character | Xenopus laevis | |
3.4.24.60 | Acetorphan | less efficient than phosphoramidon | Xenopus laevis | |
3.4.24.60 | benzamidine | not | Xenopus laevis | |
3.4.24.60 | EDTA | metalloendopeptidase character | Xenopus laevis | |
3.4.24.60 | EGTA | metalloendopeptidase character | Xenopus laevis | |
3.4.24.60 | Kelatorphan | less efficient than phosphoramidon | Xenopus laevis | |
3.4.24.60 | additional information | pepstatin, guanidylethylmercaptosuccinic acid (i.e. GEMSA), captopril, aprotinin, soybean trypsin inhibitor (i.e. STI), PMSF, IAA; retrothiorphan | Xenopus laevis | |
3.4.24.60 | N-ethylmaleimide | not | Xenopus laevis | |
3.4.24.60 | p-chloromercuriphenylsulfonic acid | not | Xenopus laevis | |
3.4.24.60 | Pentapeptides reproducing or mimicking the COOH-terminal sequence of ANF | 24-28 ANF as substrate | Xenopus laevis | |
3.4.24.60 | phosphoramidon | only at high concentrations, above 0.001 mM | Xenopus laevis | |
3.4.24.60 | thiorphan | i.e. 3-mercapto-2-benzyl-propanoylglycine, only at high concentrations, above 0.001 mM | Xenopus laevis | |
3.4.24.60 | tosyl-Arg methyl ester | not | Xenopus laevis | |
3.4.24.60 | [Leu5]enkephalin | i.e. Tyr-Gly-Gly-Phe-Leu, ANF as substrate | Xenopus laevis | |
3.4.24.60 | [Leu5]enkephalinamide | ANF as substrate | Xenopus laevis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.60 | 0.018 | - |
Somatostatin-14 | - |
Xenopus laevis | |
3.4.24.60 | 0.02 | - |
Substance P | - |
Xenopus laevis | |
3.4.24.60 | 0.021 | - |
Neuromedin B | - |
Xenopus laevis | |
3.4.24.60 | 0.022 | - |
Neuromedin C | - |
Xenopus laevis | |
3.4.24.60 | 0.024 | - |
Litorin | - |
Xenopus laevis | |
3.4.24.60 | 0.041 | - |
bradykinin | - |
Xenopus laevis | |
3.4.24.60 | 0.05 | - |
(5-28)ANF | - |
Xenopus laevis | |
3.4.24.60 | 0.063 | - |
angiotensin II | - |
Xenopus laevis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.60 | additional information | metalloendopeptidase | Xenopus laevis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.60 | 100000 | - |
1 * 100000, Xenopus laevis, SDS-PAGE | Xenopus laevis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.60 | additional information | Xenopus laevis | might be involved in inactivation of a great number of peptide hormones | ? | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.4.24.60 | Methanol | resistant to 30% v/v methanol | Xenopus laevis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.60 | Xenopus laevis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.60 | - |
Xenopus laevis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.24.60 | sweat | - |
Xenopus laevis | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.24.60 | 0.1826 | - |
[D-Arg8]-kermit as substrate | Xenopus laevis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.60 | (5-28)ANF + H2O | i.e. atrial natriuretic factor, cleavage site: exclusively Ser25-Phe26 | Xenopus laevis | ANF(5-25) + Phe26-Arg27-Tyr28 | - |
? | |
3.4.24.60 | angiotensin II + H2O | i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe, cleavage site: Tyr4-Ile5 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | bradykinin + H2O | i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg, major cleavage site: Gly4-Phe5, minor site: Pro7-Pro8 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | Litorin + H2O | minor sites: Gly7-His8 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | Litorin + H2O | major cleavage sites: His8-Phe9 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | additional information | substrate specificity | Xenopus laevis | ? | - |
? | |
3.4.24.60 | additional information | exhibits preference for cleavage on amino-terminal side of Phe, Leu or Ile in Xaa-Leu, Xaa-Phe or Xaa-Ile dipeptide | Xenopus laevis | ? | - |
? | |
3.4.24.60 | additional information | no cleavage of analogous bonds in [Leu5]-enkephalin, oxytocin, vasopressin | Xenopus laevis | ? | - |
? | |
3.4.24.60 | additional information | cleavage sites: Xaa-Phe, Xaa-Leu, Xaa-Ile | Xenopus laevis | ? | - |
? | |
3.4.24.60 | additional information | [Leu5]enkephalin amide, minigastrin I | Xenopus laevis | ? | - |
? | |
3.4.24.60 | additional information | might be involved in inactivation of a great number of peptide hormones | Xenopus laevis | ? | - |
? | |
3.4.24.60 | Neuromedin B + H2O | i.e. Gly-Asn-Leu-Trp-Ala-Thr-Gly-His-Phe-Met-NH2, major cleavage site: His8-Phe9, minor site: Gly7-His8 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | Neuromedin C + H2O | Gly-Asn-His-Trp-Ala-Val-Gly-His-Leu-Met-NH2, major cleavage site: His8-Leu9, minor site: Gly7-His8 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | Somatostatin-14 + H2O | i.e. Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys, cleavage site: Phe6-Phe7 within disulfide bridged segment yielding one single product | Xenopus laevis | ? | - |
? | |
3.4.24.60 | Substance P + H2O | major cleavage site: Gly9-Leu10, minor site: Phe7-Phe8 | Xenopus laevis | ? | - |
? | |
3.4.24.60 | [D-Arg8]-kermit + H2O | model substrate, derivative of kermit i.e. Asp-Val-Asp-Glu-Arg-Asp-Val-Arg-Gly-Phe-Ala-Ser-Phe-Leu-NH2, cleavage site: Ser12-Phe13 | Xenopus laevis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.60 | monomer | 1 * 100000, Xenopus laevis, SDS-PAGE | Xenopus laevis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.60 | 7.5 | - |
- |
Xenopus laevis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.60 | 6 | 8 | about half-maximal activity at pH 6 and about 75% of maximal activity at pH 8 | Xenopus laevis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.60 | 7.4 | - |
24 h stable | Xenopus laevis |