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Literature summary extracted from
- Purification to homogeneity of an insulin-degrading enzyme from human erythrocytes (1980), Hoppe-Seyler's Z. Physiol. Chem., 361, 1029-1039.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.24.56 | repeated freezing and thawing inactivates | Homo sapiens |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.56 | 37000 | - |
4 * 37000, human, SDS-PAGE of denatured enzyme | Homo sapiens |
| 3.4.24.56 | 150000 | 160000 | human, gel chromatography, gel electrophoresis | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.56 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.56 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.56 | erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.56 | additional information | - |
- |
Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.24.56 | -40°C, neutral pH, stable for a long time | Homo sapiens |
| 3.4.24.56 | 4°C, neutral pH, stable for several days but specific activity decreases | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.56 | Insulin + H2O | specific for insulin | Homo sapiens | Hydrolyzed insulin | degradation products are smaller than the A-chain of insulin | ? |  |
| 3.4.24.56 | additional information | fructose 1,6-bisphosphatase | Homo sapiens | ? | - |
? | |
| 3.4.24.56 | additional information | hexosephosphate isomerase | Homo sapiens | ? | - |
? | |
| 3.4.24.56 | additional information | aldolase | Homo sapiens | ? | - |
? | |
| 3.4.24.56 | additional information | no inactivation of: lactate dehydrogenase | Homo sapiens | ? | - |
? | |
| 3.4.24.56 | additional information | thyroid-stimulating hormone | Homo sapiens | ? | - |
? | |
| 3.4.24.56 | additional information | prolactin | Homo sapiens | ? | - |
? | |
| 3.4.24.56 | additional information | hexokinase | Homo sapiens | ? | - |
? | |
| 3.4.24.56 | additional information | not: growth hormone | Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.56 | tetramer | 4 * 37000, human, SDS-PAGE of denatured enzyme | Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.56 | 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.56 | 40 | - |
irreversible inactivation and denaturation | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.56 | additional information | - |
pI: 5.8 | Homo sapiens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.56 | 5.5 | 9 | unstable below pH 5.5 and above pH 9.0 | Homo sapiens |
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Release 2023.1 (January 2023)
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