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Literature summary extracted from

  • Bouvier, J.; Schneider, P.; Etges, R.
    Leishmanolysin: surface metalloproteinase of Leishmania (1995), Methods Enzymol., 248, 614-633.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.36 membrane
-
 Leishmania amazonensis 16020
-
3.4.24.36 membrane glycosylphosphatidylinositol-anchored Leishmania major 16020
-
3.4.24.36 soluble Leishmania mexicana amastigotes Leishmania amazonensis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.36 Zinc zinc containing metalloproteinase Leishmania amazonensis
3.4.24.36 Zinc zinc containing metalloproteinase Leishmania major
3.4.24.36 Zinc zinc containing metalloproteinase Leishmania infantum

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.36 Leishmania amazonensis
-
-
-
3.4.24.36 Leishmania infantum
-
-
-
3.4.24.36 Leishmania major
-
 infective strain LV39
-
3.4.24.36 Leishmania major
-
 non-infective strains LEM513
-
3.4.24.36 Leishmania major
-
 non-infective strains LRC-119
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.36 lipophosphoglycan deficient LRC-L119 promastigotes Leishmania major

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.36 amastigote
-
 Leishmania amazonensis
-
3.4.24.36 promastigote
-
 Leishmania amazonensis
-
3.4.24.36 promastigote
-
 Leishmania major
-
3.4.24.36 promastigote
-
 Leishmania infantum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.36 Ala-Arg-Ser-Val-Val-Arg-Asp-Val-Asn + H2O i.e. synthetic peptide derived from leishmanolysin Leishmania major Ala-Arg-Ser-Val + Val-Arg-Asp-Val-Asn
-
 ?
3.4.24.36 azocasein + H2O
-
 Leishmania major low molecular weight acid-soluble azopeptides
-
 ?
3.4.24.36 Casein micelles + H2O milk assay Leishmania major ?
-
 ?
3.4.24.36 Dansyl-Ala-Tyr-Leu-Lys-Lys-Trp-Val-NH2 + H2O cleavage site: Tyr-Leu Leishmania major Dansyl-Ala-Tyr + ?
-
 ?
3.4.24.36 Elastin + H2O zymographic assay Leishmania major ?
-
 ?
3.4.24.36 Fibrinogen + H2O
-
 Leishmania amazonensis ?
-
 ?
3.4.24.36 Fibrinogen + H2O ovine fibrinogen, zymographic assay Leishmania major ?
-
 ?
3.4.24.36 Glucagon + H2O
-
 Leishmania major ?
-
 ?
3.4.24.36 Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr + H2O from horse cytochrome Leishmania major Leu-Ile-Ala-Tyr + ?
-
 ?
3.4.24.36 Leu-Ile-Ala-Tyr-Ser-Lys-Lys-Ala-Thr + H2O from horse cytochrome Leishmania major ?
-
 ?
3.4.24.36 additional information specificity is defined by P'-subsites of the substrates Leishmania major ?
-
 ?
3.4.24.36 Oxidized insulin B-chain + H2O
-
 Leishmania major ?
-
 ?
3.4.24.36 Peptide 170-182 from CW-3 HLA antigen + H2O
-
 Leishmania major ?
-
 ?
3.4.24.36 Peptide 83-94 from beta-chain of HLA DO + H2O
-
 Leishmania major ?
-
 ?
3.4.24.36 Peptide 97-105 from Leishmanolysin + H2O
-
 Leishmania major ?
-
 ?
3.4.24.36 Peptides from horse cytochrome c + H2O i.e. peptides 1-25, 39-53 and 81-104 Leishmania major ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.36 37
-
 assay at Leishmania amazonensis
3.4.24.36 37
-
 assay at Leishmania major

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.36 5.5 6 soluble amastigote enzyme, Leishmania mexicana Leishmania major