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Literature summary extracted from

  • Bouvier, J.; Schneider, P.; Etges, R.; Bordier, C.
    Peptide substrate specificity of the membrane-bound metalloprotease of Leishmania (1990), Biochemistry, 29, 10113-10119.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.36 additional information possible autocatalytic mechanism for activation Leishmania sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.36 Benzyloxycarbonyl-Tyr-Leu-NHOH i.e. hydroxamate-derivatized dipeptide, strong, azocasein as substrate Leishmania sp.
3.4.24.36 additional information no inhibition by carboxymethyl derivative of Phe-Leu or hydroxamate derivatives of Leu or Tyr Leishmania sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.36 20
-
 Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr
-
 Leishmania sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.36 membrane
-
 Leishmania sp. 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.36 Zinc zinc containing metalloproteinase Leishmania sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.36 Leishmania sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.36 promastigote
-
 Leishmania sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.36 Ala-Arg-Ser-Val-Val-Arg-Asp-Val-Asn + H2O i.e. synthetic peptide derived from leishmanolysin Leishmania sp. Ala-Arg-Ser-Val + Val-Arg-Asp-Val-Asn
-
 ?
3.4.24.36 Ala-Arg-Ser-Val-Val-Arg-Asp-Val-Asn + H2O covers the last 4 amino acids of the prosequence and the first 5 amino acids of the native Leishmanolysin, cleavage site: Val-Val Leishmania sp. Ala-Arg-Ser-Val + Val-Arg-Asp-Val-Asn
-
 ?
3.4.24.36 azocasein + H2O
-
 Leishmania sp. low molecular weight acid-soluble azopeptides
-
 ?
3.4.24.36 Glucagon + H2O major cleavage sites: Tyr10-Ser11, Asp15-Ser16, minor site: Trp25-Leu26 Leishmania sp. ?
-
 ?
3.4.24.36 Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr + H2O from horse cytochrome Leishmania sp. Leu-Ile-Ala-Tyr + ?
-
 ?
3.4.24.36 Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr + H2O model peptide cytochrome c(94-102)L, cleavage site: Tyr97-Leu98 Leishmania sp. Leu-Ile-Ala-Tyr + ?
-
 ?
3.4.24.36 Leu-Ile-Ala-Tyr-Ser-Lys-Lys-Ala-Thr + H2O from horse cytochrome Leishmania sp. ?
-
 ?
3.4.24.36 Leu-Ile-Ala-Tyr-Ser-Lys-Lys-Ala-Thr + H2O model peptide cytochrome c(94-102)S, cleavage site: Tyr97-Ser98 Leishmania sp. ?
-
 ?
3.4.24.36 additional information substrate specificity Leishmania sp. ?
-
 ?
3.4.24.36 additional information preferentially cleaves at amino side when hydrophobic residues are in P1'-site, basic amino acids in P2' and P3' and commonly Tyr at P1 Leishmania sp. ?
-
 ?
3.4.24.36 Oxidized insulin B-chain + H2O major cleavage site: Gly8-Ser9, minor site: Tyr16-Leu17 Leishmania sp. ?
-
 ?
3.4.24.36 Peptide 170-182 from CW-3 HLA antigen + H2O major cleavage site: Lys75-Asn76, minor site: Tyr73-Leu74 Leishmania sp. ?
-
 ?
3.4.24.36 Peptide 83-94 from beta-chain of HLA DO + H2O cleavage site: Arg84-Leu85 Leishmania sp. ?
-
 ?
3.4.24.36 Peptide 97-105 from Leishmanolysin + H2O
-
 Leishmania sp. ?
-
 ?
3.4.24.36 Peptides from horse cytochrome c + H2O peptide 81-104 cleavage sites: Gly84-Ile85 and Tyr97-Leu98 Leishmania sp. ?
-
 ?
3.4.24.36 Peptides from horse cytochrome c + H2O i.e. peptides 1-25, 39-53 and 81-104 Leishmania sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.36 Promastigote surface protease
-
 Leishmania sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.36 37
-
 assay at Leishmania sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.36 7 8.5 Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr as substrate Leishmania sp.
3.4.24.36 7.5 10 azocasein as substrate Leishmania sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.36 4.7 11.7 about half-maximal activity at pH 4.7 and 11.7, azocasein as substrate Leishmania sp.
3.4.24.36 5.3 9.5 about half-maximal activity at pH 5.3 and 9.5, Leu-Ile-Ala-Tyr-Leu-Lys-Lys-Ala-Thr as substrate Leishmania sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.36 4 11 at least 30 min stable Leishmania sp.