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Literature summary extracted from

  • Inagaki, T.; Tadasa, K.; Kayahara, H.
    Modification effects of organic solvents on microenvironment of the enzyme in thermolysin-catalyzed peptide synthesis of N-(benzyloxycarbonyl)-L-phenylalanyl-L-phenylalanine methyl ester (1994), Biosci. Biotechnol. Biochem., 58, 1439-1442.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.27 additional information direct effect of organic solvents on the microenvironment of the enzyme largely depends on the molecular structure of the solvent Bacillus thermoproteolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.27 39.2
-
 N-(benzyloxycarbonyl)-L-Phe
-
 Bacillus thermoproteolyticus
3.4.24.27 250
-
 L-Phe methyl ester pH 5.5 or 6.0 Bacillus thermoproteolyticus
3.4.24.27 300
-
 L-Phe methyl ester
-
 Bacillus thermoproteolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.27 Bacillus thermoproteolyticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.27 N-(Benzyloxycarbonyl)-L-Phe + L-Phe methyl ester
-
 Bacillus thermoproteolyticus N-(Benzyloxycarbonyl)-L-Phe-L-Phe methyl ester + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.27 1.69
-
 N-(benzyloxycarbonyl)-L-Phe
-
 Bacillus thermoproteolyticus