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Literature summary extracted from

  • Baricos, W.H.; Murphy, G.; Zhou, Y.; Nguyen, H.H.; Shah, S.V.
    Degradation of glomerular basement membrane by purified mammalian metalloproteinases (1988), Biochem. J., 254, 609-612.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.4.24.17 Ca2+ stabilizes Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.17 1,10-phenanthroline
-
 Homo sapiens
3.4.24.17 EDTA
-
 Homo sapiens
3.4.24.17 additional information not: inhibitors for serine, cysteine or aspartic proteinases; phosphoramidon Homo sapiens
3.4.24.17 NEM
-
 Homo sapiens
3.4.24.17 U24522 synthetic inhibitor known to inhibit proteoglycan-degrading metalloproteinases Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.17 0.27
-
 acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly-ethylester
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.17 Homo sapiens
-
 expression in Escherichia coli
-
3.4.24.17 Homo sapiens
-
 catalytic domain lacking both propeptide and C-terminal fragment
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.17 catalytic domain lacking both propeptide and C-terminal fragment Homo sapiens
3.4.24.17 expression in Escherichia coli Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.17 additional information
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.4.24.17 4°C, 50 mM Tris-HCl, pH 7.6, 10 mM CaCl2, stable Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.17 Acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly ethyl ester + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.17 Proteoglycan + H2O
-
 Homo sapiens More
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.17 37
-
 stable at pH 6-9 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.17 2.12
-
 Acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly ethyl ester
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.17 6
-
 acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly-ethylester Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.17 5 7.5 pH 5.0: about 20% of activity maximum, pH 7.5: about 25% of activity maximum Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.17 6 9 37°C, stable Homo sapiens