Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Murao, S.; Funakoshi, S.; Oda, K.
    Acid protease of Cladosporium species No. 45-2. I. Purification, crystallization, and some enzymic properties of acid protease of Cladosporium species No. 45-2 (1972), Agric. Biol. Chem., 36, 1327-1333.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.23.26
-
 Cladosporium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.26 Hg2+ weak Cladosporium sp.
3.4.23.26 Ni2+ weak Cladosporium sp.
3.4.23.26 PCMB weak Cladosporium sp.
3.4.23.26 sodium lauryl sulfate
-
 Cladosporium sp.
3.4.23.26 Streptomyces-pepsin inhibitor most resistant towards Streptomyces-pepsin inhibitor among the acid proteases tested Cladosporium sp.
3.4.23.26 sulfhydryl reagents
-
 Cladosporium sp.
3.4.23.26 Zn2+ weak Cladosporium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.26 Cladosporium sp.
-
 No. 45-2
-
3.4.23.26 Cladosporium sp. No. 45-2
-
 No. 45-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.26 No. 45-2 Cladosporium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.23.26 additional information
-
-
 Cladosporium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.26 casein + H2O
-
 Cladosporium sp. ?
-
 ?
3.4.23.26 casein + H2O
-
 Cladosporium sp. No. 45-2 ?
-
 ?
3.4.23.26 Hemoglobin + H2O
-
 Cladosporium sp. ?
-
 ?
3.4.23.26 Hemoglobin + H2O
-
 Cladosporium sp. No. 45-2 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.23.26 55
-
-
 Cladosporium sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.23.26 30 65 30°C and 65°C: about 40% of activity maximum Cladosporium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.23.26 30
-
 pH 2.5-7.0, 20 h, stable Cladosporium sp.
3.4.23.26 70
-
 pH 2.5, 10 min, loss of activity Cladosporium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.26 2.5 2.7 casein, hemoglobin Cladosporium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.23.26 1.5 6 about 25% of activity maximum at pH 1.5 and 6 Cladosporium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.23.26 2.5
-
 70°C, 10 min, loss of activity Cladosporium sp.
3.4.23.26 2.5 7 30°C, 20 h, stable Cladosporium sp.