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Literature summary extracted from

  • Iio, K.; Yamasaki, M.
    Specificity of acid proteinase A from Aspergillus niger var. macrosporus towards B-chain of performic acid oxidized bovine insulin (1976), Biochim. Biophys. Acta, 429, 912-924.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.18 additional information diisopropyl phosphofluoridate Aspergillus niger
3.4.23.18 pepstatin
-
 Aspergillus niger
3.4.23.19 1,2-epoxy-3-(4-nitrophenoxy)propane not Aspergillus niger
3.4.23.19 Diazoacetyl-DL-norleucine methyl ester in the presence of Cu2+ Aspergillus niger
3.4.23.19 additional information no inhibition by diisopropyl fluorophosphate; no inhibition by pepstatin Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.18 Aspergillus niger
-
 var. macrosporus
-
3.4.23.19 Aspergillus niger
-
 var. macrosporus
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.23.19 commercial preparation proctase powder, crude enzyme powder from waters of Koji cultures Aspergillus niger
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.18 casein + H2O
-
 Aspergillus niger ?
-
 ?
3.4.23.19 casein + H2O
-
 Aspergillus niger hydrolyzed casein
-
 ?
3.4.23.19 Oxidized insulin B-chain + H2O major cleavage sites: Asn3-Gln4, Glu13-Ala14, Tyr26-Thr27, minor site: Gly20-Glu21 Aspergillus niger Hydrolyzed insulin B-chain
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.23.18 30
-
 assay at Aspergillus niger
3.4.23.19 30
-
 assay at Aspergillus niger