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Literature summary extracted from

  • Baumgartner, B.; Chrispeels, M.J.
    Purification and characterization of vicilin peptidohydrolase, the major endopeptidase in the cotyledons of mung-bean seedlings (1977), Eur. J. Biochem., 77, 223-233.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.34 2-mercaptoethanol 10 mM, 300% activation Vigna radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.34 additional information not: EDTA; N-tosyl-L-lysine chloromethyl ketone; phenylmethylsulfonyl fluoride Vigna radiata
3.4.22.34 NEM
-
 Vigna radiata
3.4.22.34 PCMB
-
 Vigna radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.34 22500
-
 1 * 22500, Vigna radiata, SDS-PAGE Vigna radiata
3.4.22.34 23500
-
 Vigna radiata, gel filtration Vigna radiata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.34 Vicilin + H2O Vigna radiata degradation of the principal reserve protein present in Vigna radiata seeds ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.34 Vigna radiata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.34
-
 Vigna radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.34 cotyledon
-
 Vigna radiata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.34 additional information
-
-
 Vigna radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.34 Benzyloxycarbonyl-Ala 4-nitrophenyl ester + H2O
-
 Vigna radiata Benzyloxycarbonyl-Ala + 4-nitrophenol
-
 ?
3.4.22.34 Benzyloxycarbonyl-Asn nitrophenyl ester + H2O
-
 Vigna radiata Benzyloxycarbonyl-Asn + 4-nitrophenol
-
 ?
3.4.22.34 Benzyloxycarbonyl-Gly 4-nitrophenyl ester + H2O
-
 Vigna radiata Benzyloxycarbonyl-Gly + 4-nitrophenol
-
 ?
3.4.22.34 benzyloxycarbonyl-Phe 4-nitrophenyl ester + H2O
-
 Vigna radiata benzyloxycarbonyl-Phe + 4-nitrophenol
-
 ?
3.4.22.34 Benzyloxycarbonyl-Tyr 4-nitrophenyl ester + H2O
-
 Vigna radiata Benzyloxycarbonyl-Tyr + 4-nitrophenol
-
 ?
3.4.22.34 tert-butoxycarbonyl-Asn nitrophenyl ester + H2O
-
 Vigna radiata tert-butoxycarbonyl-Asn + 4-nitrophenol
-
 ?
3.4.22.34 Tert-Butoxycarbonyl-Gln 4-nitrophenyl ester + H2O
-
 Vigna radiata Tert-Butoxycarbonyl-Gln + 4-nitrophenol
-
 ?
3.4.22.34 Vicilin + H2O
-
 Vigna radiata Hydrolyzed vicilin
-
 ?
3.4.22.34 Vicilin + H2O degradation of the principal reserve protein present in Vigna radiata seeds Vigna radiata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.34 monomer 1 * 22500, Vigna radiata, SDS-PAGE Vigna radiata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.34 35
-
 assay at Vigna radiata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.34 35
-
 10 min, stable up to Vigna radiata
3.4.22.34 50
-
 10 min, complete inactivation Vigna radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.34 5.1
-
 vicilin Vigna radiata