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Literature summary extracted from

  • Harrach, T.; Eckert, K.; Schulze-Forster, K.; Nuck, R.; Grunow, D.; Maurer, H.R.
    Isolation and partial characterization of basic proteinases from stem bromelain (1995), J. Protein Chem., 14, 41-52.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.32 0.4
-
 L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide enzyme form F9 Ananas comosus
3.4.22.32 2.3
-
 L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide enzyme form F4 Ananas comosus
3.4.22.32 2.42
-
 L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide enzyme form F5 Ananas comosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.32 23430
-
 Ananas comosus, enzyme form F9, mass spectroscopy Ananas comosus
3.4.22.32 24400
-
 Ananas comosus, enzyme form F4, mass spectroscopy Ananas comosus
3.4.22.32 24470
-
 Ananas comosus, enzyme form F5, mass spectroscopy Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus
-
 at least 8 basic proteolytically active components
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.32 glycoprotein enzyme form F4 and F5 contain fucose, N-acetylglucosamine, xylose and mannose in the ratio of 1.0:2.0:1.0:2.0, but only 50% of the proteins seem to be glycosylated, enzyme form F9 is unglycosylated Ananas comosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.32 stem
-
 Ananas comosus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.32 L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide + H2O
-
 Ananas comosus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.32 0.68
-
 L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide enzyme form F5 Ananas comosus
3.4.22.32 3.94
-
 L-Pyroglutamyl-L-Phe-L-Leu 4-nitroanilide enzyme form F9 Ananas comosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.32 4 4.5 L-pyroglutamyl-L-Phe-L-Leu 4 nitroanilide, enzyme form F4 and F5 Ananas comosus
3.4.22.32 7
-
 about, L-pyroglutamyl-L-Phe-L-Leu 4-nitroanilide, enzyme form F9 Ananas comosus