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Literature summary extracted from

  • Napper, A.D.; Bennett, S.P.; Borowski, M.; Holridge, M.B.; Leonard, M.J.C.; Rogers, E.E.; Duan, Y.; Laursen, R.A.; Reinhold, B.; Shames, S.L.
    Purification and characterization of multiple forms of the pineapple-stem-derived cysteine proteinases ananain and comosain (1994), Biochem. J., 301, 727-735.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.31 L-3-carboxy-2,3-trans-epoxypropionylleucylamido(4-guanidino)butane i.e. E-64 Ananas comosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.31 1.6
-
 benzoyl-Phe-Val-Arg 4-nitroanilide
-
 Ananas comosus
3.4.22.31 11
-
 benzyloxycarbonyl-Arg-Arg 4-nitroanilide
-
 Ananas comosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.31 23420
-
 Ananas comosus, electrospray mass spectrometry Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.31 Ananas comosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.31
-
 Ananas comosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.31 stem
-
 Ananas comosus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.31 Benzoyl-Phe-Val-Arg 4-nitroanilide + H2O
-
 Ananas comosus ?
-
 ?
3.4.22.31 Benzyloxycarbonyl-Arg-Arg 4-nitroanilide + H2O
-
 Ananas comosus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.31 0.13
-
 benzyloxycarbonyl-Arg-Arg 4-nitroanilide
-
 Ananas comosus
3.4.22.31 10.9
-
 benzoyl-Phe-Val-Arg 4-nitroanilide
-
 Ananas comosus