EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.31 | chicken cystatin | strong | Ananas comosus | |
3.4.22.31 | L-3-carboxy-2,3-trans-epoxypropionylleucylamido(4-guanidino)butane | i.e. E-64 | Ananas comosus | |
3.4.22.32 | L-3-carboxy-2,3-trans-epoxypropionylleucylamido(4-guanidino)butane | irreversible | Ananas comosus | |
3.4.22.32 | additional information | scarcely inhibited by chicken cystatin (amino acid sequence and implications for weak binding of cystatin) | Ananas comosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.31 | 0.0131 | - |
benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide | - |
Ananas comosus | |
3.4.22.32 | 0.0154 | - |
benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide | - |
Ananas comosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.31 | Ananas comosus | - |
- |
- |
3.4.22.32 | Ananas comosus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.22.31 | additional information | no glycoprotein | Ananas comosus |
3.4.22.32 | glycoprotein | - |
Ananas comosus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.31 | - |
Ananas comosus |
3.4.22.32 | - |
Ananas comosus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.22.31 | stem | - |
Ananas comosus | - |
3.4.22.32 | stem | - |
Ananas comosus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.22.32 | additional information | - |
assay methods | Ananas comosus |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.22.31 | -20°C, stored dry, stable | Ananas comosus |
3.4.22.32 | -20°C, stored dry, stable | Ananas comosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.31 | azocasein + H2O | - |
Ananas comosus | fragments of azocasein | - |
? | |
3.4.22.31 | Benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide + H2O | - |
Ananas comosus | Benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.22.31 | Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O | - |
Ananas comosus | Benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.22.31 | hide powder azure + H2O | - |
Ananas comosus | hydrolyzed hide powder azure | - |
? | |
3.4.22.32 | Benzoyl-Arg-Arg 4-nitrophenyl ester + H2O | - |
Ananas comosus | Benzoyl-Arg-Arg + 4-nitrophenol | - |
? | |
3.4.22.32 | Benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide + H2O | - |
Ananas comosus | Benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.22.32 | Benzyloxycarbonyl-Arg-Arg 4-nitrophenyl ester + H2O | - |
Ananas comosus | Benzyloxycarbonyl-Arg-Arg + 4-nitrophenol | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.31 | 63.8 | - |
benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide | - |
Ananas comosus | |
3.4.22.32 | 27 | - |
benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide | - |
Ananas comosus |